A bacterial product may comprise strains Azospirillum oryzae AO.sub.512, Azospirillum lipoferum AL.sub.20, Pantoea dispersa PD.sub.2, Bacillus subtilis TU.sub.2, Lysinibacillus sphaericus 3S and Paenibacillus polymyxa SR.sub.17, in a total minimal concentration of 10.sup.8 CFU/g (Colony forming Units). These bacteria may be attached to an insoluble granular carrier formed of a mixture of natural clays and minerals. This formulation may preserve the viability of the microorganism for a long time while in storage, as well as after its application on the field. These microorganisms, as PGPR, may be capable of fixing atmospheric nitrogen, solubilize phosphates, potassium, as well as other minerals immobilized in the soil, which allows for a drastic reduction, and even for a complete elimination, of conventional fertilization methods. These bacteria are not harmful in any sense neither to human beings nor the environment.

A bacterial product may comprise strains Azospirillum oryzae AO.sub.512, Azospirillum lipoferum AL.sub.20, Pantoea dispersa PD.sub.2, Bacillus subtilis TU.sub.2, Lysinibacillus sphaericus 3S and Paenibacillus polymyxa SR.sub.17, in a total minimal concentration of 10.sup.8 CFU/g (Colony forming Units). These bacteria may be attached to an insoluble granular carrier formed of a mixture of natural clays and minerals. This formulation may preserve the viability of the microorganism for a long time while in storage, as well as after its application on the field. These microorganisms, as PGPR, may be capable of fixing atmospheric nitrogen, solubilize phosphates, potassium, as well as other minerals immobilized in the soil, which allows for a drastic reduction, and even for a complete elimination, of conventional fertilization methods. These bacteria are not harmful in any sense neither to human beings nor the environment.

The present invention relates to a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain comprising a variant fusaricidin synthetase lacking a functional adenylation domain in the third module. The present invention also provides a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain or a cell-free extract thereof comprising at least one Paeniserine and at least one Paeniprolixin. Also provided are isolated compounds and methods of treating a plant to control a plant disease with the disclosed compositions and compounds.

The present invention relates to a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain comprising a variant fusaricidin synthetase lacking a functional adenylation domain in the third module. The present invention also provides a composition comprising a biologically pure culture of a fungicidal Paenibacillus sp. strain or a cell-free extract thereof comprising at least one Paeniserine and at least one Paeniprolixin. Also provided are isolated compounds and methods of treating a plant to control a plant disease with the disclosed compositions and compounds.

The disclosure provides a method of treating, preventing, and/or controlling plant diseases by applying the agricultural composition comprising a bacterial isolate belonging to Paenibacillus or Bacillus. Also, the disclosure provides a method of enhancing disease resistance of a plant seed by applying the agricultural composition to the plant seed, and an agricultural composition comprising the bacterial isolate.

The disclosure provides a method of treating, preventing, and/or controlling plant diseases by applying the agricultural composition comprising a bacterial isolate belonging to Paenibacillus or Bacillus. Also, the disclosure provides a method of enhancing disease resistance of a plant seed by applying the agricultural composition to the plant seed, and an agricultural composition comprising the bacterial isolate.

Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL pesticidal proteins are also provided.

Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL pesticidal proteins are also provided.

A treated biochar comprising a porous carbonaceous particle that has been treated and mixed with a media containing a mineral solubilizing microorganism, whereby the porous carbonaceous particle after mixing has retained the mineral solubilizing microorganism.

A treated biochar comprising a porous carbonaceous particle that has been treated and mixed with a media containing a mineral solubilizing microorganism, whereby the porous carbonaceous particle after mixing has retained the mineral solubilizing microorganism.