A method for mixing a biological suspension includes disposing a biological suspension within a compartment of a container, the biological suspension comprising cells or microorganisms suspended within a nutrient growth medium; and rotating a first drive line and laterally spaced apart second drive line within the compartment of the container so as to cause the drive lines to twist into a helical configuration and mix the biological suspension.

A stirring device has at least one at least substantially metallic stirring blade carrier and a plurality of stirring blades, which are connected to the stirring blade carrier, wherein the stirring blades are at least substantially made of a non-metallic material, wherein the non-metallic material is a ceramic material, wherein the stirring blades are releasably connected to the stirring blade carrier, wherein it is possible to release and/or establish a mechanical, namely a negative-fit and/or positive-fit connection between the stirring blades and the stirring blade carrier in a damage-free and/or non-destructive manner, and wherein the stirring blade carrier comprises at least one recess, which is configured to at least partly accommodate at least one of the stirring blades. The recess comprises at least one partial region implemented in a contiguous fashion if viewed in parallel to a rotational axis of the stirring blade carrier.

The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures.

Provided is a continuous reaction apparatus which can precisely control the path of flow of the liquid reaction mixture in the reaction vessel. Further, provided is a continuous reaction apparatus which can efficiently mix the liquid reaction mixture in the reaction vessel. The continuous reaction apparatus comprises a plurality of mixing vessel units and a plurality of partition units. These units are connected in the state of being alternately stacked on one another. Each mixing vessel unit has an agitating blade disposed in the inner space thereof. The relationship between the inner diameter D1 of the mixing vessel unit, the height H of the mixing vessel unit, and the outer diameter d1 of the agitating blade satisfies the formula (1): 10(D1/H)1.5, and the formula (2): 0.99(d1/D1)0.7. The agitating blade is a circular disc-type agitating blade.

In a rapid stirrer that stirs sludge and a flocculant in a tank with a stirring blade, the rotation speed of the stirring blade is increased at a set rate with an increase in the amount of charged sludge, the rotation speed of the stirring blade is reduced at the set rate with a decrease in the amount of charged sludge, and flocculated flocs having a fixed floc diameter are stably generated even when operating conditions change so as to fluctuate the amount of charged sludge.

The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures.

The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures.

A fluid mixing system includes a collapsible container bounding a compartment and extending between a first end and an opposing second end. An elongated continuous drive line is at least partially disposed within the compartment of the container. A first portion of the drive line is laterally spaced apart from a second portion of the drive line, and the first portion of the drive line and the second portion of the drive line are rotatable within the compartment of the container.

A fluid mixing system includes a collapsible container bounding a compartment and extending between a first end and an opposing second end. An elongated continuous drive line is at least partially disposed within the compartment of the container. A first portion of the drive line is laterally spaced apart from a second portion of the drive line, and the first portion of the drive line and the second portion of the drive line are rotatable within the compartment of the container.

A mixing system for mixing a liquid includes a first impeller segment having a first mount and a first mixing blade secured to the first mount and a second impeller segment having a second mount and a first mixing blade secured to the second mount, the second impeller segment being separate and discrete from the first impeller segment. One or more drive members are secured to the first impeller segment and the second impeller segment for concurrently rotating the first impeller segment and the second impeller segment about a rotational axis. The first impeller segment and the second impeller segment are secured to the one or more drive members so that a plane extending normal to the axis of rotation intersects with the first mixing blade of the first impeller segment and the first mixing blade of the second impeller segment.