A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

A stirring blade includes a first member having a first flat surface and a second member having a second flat surface, wherein the stirring blade is rotatable about a rotational axis in a state where the first and second flat surfaces are opposed to each other and spaced by a first distance across the rotational axis. When the stirring blade is rotated about the rotational axis with the first and second flat surfaces at least partially immersed in an object to be stirred, the object to be stirred having entered between the first and second flat surfaces is discharged in a direction away from the rotational axis by a centrifugal force and, simultaneously, the object to be stirred is sucked into and between the first and second flat surfaces from a direction along the rotational axis.

A stirring blade includes a first member having a first flat surface and a second member having a second flat surface, wherein the stirring blade is rotatable about a rotational axis in a state where the first and second flat surfaces are opposed to each other and spaced by a first distance across the rotational axis. When the stirring blade is rotated about the rotational axis with the first and second flat surfaces at least partially immersed in an object to be stirred, the object to be stirred having entered between the first and second flat surfaces is discharged in a direction away from the rotational axis by a centrifugal force and, simultaneously, the object to be stirred is sucked into and between the first and second flat surfaces from a direction along the rotational axis.

A method of mixing a fluid includes at least partially unfolding a collapsible bag bounding a compartment, the collapsible bag containing in the compartment at least a portion of an elongated drive line or drive shaft and an impeller secured to the drive line or drive shaft, the impeller including a plurality of impeller blades that are pivotable relative to the drive line or drive shaft, at least one of the plurality of impeller blades being in a collapsed position. A fluid is delivered into the compartment of the collapsible bag. The drive line or drive shaft is then rotated so as to rotate the impeller within the compartment and mix the fluid therein, the at least one of the plurality of impeller blades pivoting from the collapsed position to an expanded position as the impeller is rotated within the compartment.

A method of mixing a fluid includes at least partially unfolding a collapsible bag bounding a compartment, the collapsible bag containing in the compartment at least a portion of an elongated drive line or drive shaft and an impeller secured to the drive line or drive shaft, the impeller including a plurality of impeller blades that are pivotable relative to the drive line or drive shaft, at least one of the plurality of impeller blades being in a collapsed position. A fluid is delivered into the compartment of the collapsible bag. The drive line or drive shaft is then rotated so as to rotate the impeller within the compartment and mix the fluid therein, the at least one of the plurality of impeller blades pivoting from the collapsed position to an expanded position as the impeller is rotated within the compartment.

A thermokinetic mixer for melt mixing plastics waste includes a housing enclosing a mixing chamber, and a shaft protruding through the mixing chamber and connectable to a drive unit. In the mixing chamber, Y-shaped mixing blades protrude radially from the shaft, wherein the free end of the mixing blades protruding into the mixing chamber is cuboid, and the end opposite the free end of the mixing blades has two legs in each case having at least one through bore. The shaft has polygonal recesses, in which recesses the legs can be fastened by a fastener protruding through the through bores.

A thermokinetic mixer for melt mixing plastics waste includes a housing enclosing a mixing chamber, and a shaft protruding through the mixing chamber and connectable to a drive unit. In the mixing chamber, Y-shaped mixing blades protrude radially from the shaft, wherein the free end of the mixing blades protruding into the mixing chamber is cuboid, and the end opposite the free end of the mixing blades has two legs in each case having at least one through bore. The shaft has polygonal recesses, in which recesses the legs can be fastened by a fastener protruding through the through bores.

A method and a system for sequestering carbon dioxide (CO.sub.2) while producing freshwater are provided. An exemplary method includes producing saline water from a saline aquifer, desalinating at least a portion of the saline water, producing freshwater and waste brine, mixing waste CO.sub.2 with the waste brine forming a brine/CO.sub.2 mixture, and injecting the brine/CO.sub.2 mixture into the saline aquifer.

A caffeine reduction apparatus includes: a main housing provided with an inlet and outlet pipe; a mixing portion including an accommodating portion through which coffee grounds are input inside the accommodating portion, and a blade configured to be rotated inside the accommodating portion such that the coffee grounds are mixed; a driving portion configured to rotate the blade; and an ultraviolet ray emitting portion positioned at an upper portion of the main housing and mounted at an inner side surface of a door that seals an inner portion of the mixing portion, the ultraviolet ray emitting portion being configured to emit ultraviolet rays toward the mixing portion.

A caffeine reduction apparatus includes: a main housing provided with an inlet and outlet pipe; a mixing portion including an accommodating portion through which coffee grounds are input inside the accommodating portion, and a blade configured to be rotated inside the accommodating portion such that the coffee grounds are mixed; a driving portion configured to rotate the blade; and an ultraviolet ray emitting portion positioned at an upper portion of the main housing and mounted at an inner side surface of a door that seals an inner portion of the mixing portion, the ultraviolet ray emitting portion being configured to emit ultraviolet rays toward the mixing portion.