A mixer for mixing contents of a vessel includes a rotational mechanism with an attached gearbox. First and second shafts are coaxial with each other and attached to the gearbox. The gearbox is configured to rotate the first shaft and the second shaft in opposite directions. First and second rotors are rotated, respectively, in opposite directions by the first and second shafts. A housing encloses the gearbox and extends downward from the gearbox to just above the two contra-rotating first and second rotors (or impellers). The housing directs air (or other gas, liquid, or fluid) downward. Openings at the top of the housing admit air which is forced to the bottom of the housing by additional air impellers fastened to the outer of the two shafts. Lower housing openings allow aerated air to exit into desired material, such as fluid or solid/liquid mixture.

A fluid mixing system includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment. A first rotational assembly includes a first casing mounted to the first end of the flexible bag and a first hub rotatably mounted to the first casing. A second rotational assembly includes a second casing mounted to the second end of the flexible bag and a second hub rotatably mounted to the second casing. An elongated member has a first end coupled with the first hub and an opposing second end coupled with the second hub, the connector being flexible and having a uniform flexibility along its entire length. A first impeller is mounted to the connector.

A fluid mixing system includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment; a retainer coupled to the second end of the flexible bag, the retainer having a retention cavity formed thereon that communicates with the compartment of the flexible bag; and an elongated member having a first end rotatably coupled to the first end of the flexible bag and an opposing second end freely disposed within the retention cavity of the retainer so that the second end of the elongated member can rotate within the retention cavity relative to the retainer.

The present invention provides a stirrer set, comprising: a fitting tube having an opening portion and a hollow rotating shaft fitted into the opening portion, the hollow rotating shaft having an external wall which is annularly recessed to form at least one annular groove. The present invention also provides a stirring device, wherein a drive element comprising a power transmission element, such as a belt, may be rotatably driven to cause, via the power transmission element, the stirrer set to rotate for performing a stirring function. As such, the stirring device provides higher design flexibility when dealing with high-flux samples, and the element replacement rates can be significantly reduced. Thus, the costs related to manufacturing, repair, maintenance and other processes can be reduced in an effective manner.

The present invention is based in part on the discovery of significant improvements to cell culture systems and methods of generating organoids. The system of the invention provides a novel spinning bioreactor platform for higher-throughput 3D culturing of stem cells (e.g. human induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs)). The system can be widely used as a standard platform to generate stem cell-derived human organoids for any tissue and for high-throughput drug screenings, toxicity testing, and modeling normal human organ development and diseases.

A fluid mixing system includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment. A first rotational assembly includes a first casing mounted to the first end of the flexible bag and a first hub rotatably mounted to the first casing. A second rotational assembly includes a second casing mounted to the second end of the flexible bag and a second hub rotatably mounted to the second casing. A connector has a first end coupled with the first hub and an opposing second end coupled with the second hub, the connector being flexible and having a uniform flexibility along its entire length. A first impeller is mounted to the connector.

The present disclosure relates to a mixing assembly for a fluid mixing system. The mixing assembly includes a first rotational assembly having a first casing and a first hub rotatably mounted to the first casing, a second rotational assembly having a second casing and a second hub rotatably mounted to the second casing, an elongated member having a first end coupled with the first hub and an opposing second end coupled with the second hub, and one or more impellers secured to the elongated member.

Methods and apparatus for the treatment of animal waste are disclosed, together with a treated animal waste and fertilizer and growth media products derived therefrom.

A fluid mixing system includes a flexible bag having an interior surface bounding a compartment. A retainer is coupled to the flexible bag, the retainer having a retention cavity formed thereon that communicates with the compartment of the flexible bag. An elongated tubular connector has a first end coupled to the flexible bag and an opposing second end projecting into the compartment thereof. An impeller is disposed within flexible bag and is coupled with the tubular connector. A steady support is mounted on the second end of the tubular connector and terminates at a distal nose, the distal nose being freely disposed within the retention cavity of the retainer so that the steady support can rotate within the retention cavity relative to the retainer.

Methods and apparatus for the treatment of animal waste are disclosed, together with a treated animal waste and fertilizer and growth media products derived therefrom.