The invention relates to an emulsification device for continuously producing emulsions, nano-emulsions, and/or dispersions having a liquid crystalline structure, comprising a) at least one mixing system, b) at least one drive for the stirring element, and c) at least one delivery unit for each component or each component mixture.

A sanitary mixing tank steady bearing is replaceable without lifting a mixing tank shaft. The steady bearing is insertable into and removable from the bottom of a bearing support attached inside the base of the sanitary mixing tank. The steady bearing may thus be replaced by a service technician working through a man way/man hole on the top of the tank allowing service without lifting the mixing tank shaft. Gaps are provided between the bearing support and steady bearing allowing flushing material from around the steady bearing to easily maintain a sanitary environment.

A conical mixer with two conical bodies that are sloped downward toward a discharge opening on a bottom, middle portion of the conical mixer. The conical mixer has a horizontally placed agitator axle with a plurality of agitator arms extending from the agitator axle. At least one paddle is attached to each agitator arms. As the agitator arms get closer to the middle, the length of the arms is increased. The conical mixer ensures proper mixing of the contents as well as using the slope of the conical bodies to assist in pushing the contents toward the discharge opening.

A fluid transfer assembly for single use bioreactors includes a fluid transfer housing that can be mounted to the impeller shaft using a bearing that places the fluid transfer assembly directly below the lowest impeller but allows the impeller shaft to spin inside independently of the fluid transfer assembly. A fluid conduit connects the fluid transfer housing to a port in the single use bag wall which allows fluids to be introduced into the sparger and which also helps prevent the fluid transfer assembly from rotating with the impeller shaft.

A fluid mixing system includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment; a retainer coupled to the second end of the flexible bag, the retainer having a retention cavity formed thereon that communicates with the compartment of the flexible bag; and an elongated member having a first end rotatably coupled to the first end of the flexible bag and an opposing second end freely disposed within the retention cavity of the retainer so that the second end of the elongated member can rotate within the retention cavity relative to the retainer.

The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.

The present set of embodiments relate to a bioproduction system, method, and apparatus for mixing a fluid. The bioproduction mixing system includes an offset helical assembly having a stabilizer and impeller for mixing a fluid within a flexible compartment The bioproduction mixing system is designed for efficient mixing of the fluid and for use with a variety of different impellers that can be located at different locations according to the volume and shape characteristics of the flexible compartment. The bioproduction mixing system is optimized to eliminate stagnation zones while maximizing bulk fluid flow.

The present set of embodiments relate a system, method, and various devices for installation of a bioproduction system. The bioproduction system includes a rigid housing having a moveable platform to assist in the installation of a bioprocessing container within. The system also includes mounting systems for a variety of peripherals originating from the flexible container while safeguarding operators. More specifically, the system includes systems and methods for mounting bearings, organizing tube sets, and placement of the flexible container in its proper orientation.

The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

A fluid mixing system includes a container, such as a flexible bag, bounding a compartment. A flexible drive line is disposed within the compartment, the drive line having a first end rotatably connected to a first end of the container and an opposing second end rotatably connected to a second end of the container. At least one mixing element, such as an impeller, can be coupled with the flexible drive line. Rotation of the drive line facilitates rotation of the impeller within the container.