A dilution device may include a first component and a second component. The first component may define a groove including an inlet portion and an outlet portion. The second component may define an inlet in fluid communication with the inlet portion of the first component and an outlet in fluid communication with the outlet portion of the first component. Relative rotation between the first component and the second component may cause relative movement between the outlet and the outlet portion that changes the effective length of the groove fluidly coupling the inlet and the outlet of the second component. The cross-sectional area of the groove may vary along a length of the groove to provide different flow characteristics depending on the effective length of the groove.

Gas injection apparatuses include a gas inlet, a gas outlet, a primary gas chamber, a gas reduction chamber, and a fluid dispensing passageway. The primary gas chamber has a first cross sectional size and is fluidly connected to the gas inlet and the gas outlet. The gas reduction chamber is fluidly connected to the primary gas chamber, the gas inlet, and the gas outlet. The gas reduction chamber has a second cross sectional size that is less than the first cross sectional size. The fluid dispensing passageway is fluidly connected to the gas reduction chamber by a gas delivery orifice having a third cross sectional size that is less than the second cross sectional size.

A pressurized fluid mixing device is disclosed, including an inner casing and an outer casing. A first channel is arranged in the inner casing and includes one or more unit channels, adjacent unit channels of which are communicated with each other, flow blocking members are fixed on the unit channels, the inner casing is provided with one or more first inlets and one or more first outlets, a second channel is arranged in the outer casing, the outer casing is provided with one or more second inlets and one or more second outlets, and the inner casing is fixed on the second channel.

A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

An agitator is capable of more efficiently applying a shear force to a treatment target fluid through the action of an intermittent jet flow. The agitator includes a rotor which includes blade, and a screen concentric therewith, the rotation of at least the rotor causing a treatment target fluid to pass through slits in the screen to become an intermittent get stream, which is discharged from inside the screen to the outside thereof, wherein the agitator is characterized in that: the screen has a cylindrical shape with a circular cross section; openings of the slits provided in an inner surface of the screen constitute flow intake openings; openings of the slits provided in an outer surface of the screen constitute flow discharge openings; spaces between the flow intake openings and the flow discharge openings constitute slit space; and the circumferential-directional width (So) of the flow discharge openings and the circumferential-directional width (Si) of the flow intake openings are greater than the circumferential-directional width (Sm) of the slit spaces.

A vacuum deaerator degasses material to be processed by placing a rotating rotor with a screen in a vacuum vessel, introducing a liquid material to be processed into the rotor from the interior thereof and causing the liquid to pass through the screen to refine the same. The vacuum deaerator is characterized in that: the screen is a cylinder with a circular cross-section and is in the form of a porous plate in which a plurality of through holes are opened in the radial direction of the cylindrical screen; and the screen is provided such that the area of inflow openings is greater than the area of outflow openings, where the inflow openings are openings of a plurality of penetration portions provided on the inner wall face of the screen and the outflow openings are openings of the plurality of penetration portions provided on the outer wall dace of the screen. Thus, the processing capacity of the vacuum deaerator is improved without increasing the size of the device.

A microscale bioreactor system for and method is disclosed for providing improved cell culture growth conditions in a small-volume vessel. For example, a microbioreactor system is provided that may include a small-volume vessel and wherein the small-volume vessel may include a field of actuatable surface-attached microposts. Further, the microbioreactor system may include an actuation mechanism for actuating the surface-attached microposts into movement. In some embodiments, the surface-attached microposts may be functionalized with, for example, activation signals for converting standard T-cells in a growth media to activated T-cells. Further, a method of using the microbioreactor system for providing cell culture growth conditions including enhanced oxygenation and nutrients distribution in a small-volume vessel is provided.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.

A channel manifold is presented, and which includes a dimensionally stable channel plate having top, bottom and side surfaces and a channel formed in a surface thereof, wherein the channel splits into a plurality of subsidiary channels along the surface of the channel plate and has an entrance port and a plurality of exit ports such that a fluid fed into the entrance port is expelled through the plurality of exit ports.

A method for manufacturing slurry for an insulation protective layer of a rechargeable battery includes obtaining an insulation material calibration curve showing a relationship between particle size and compressibility of an insulation material using sets of particle size and compressibility of the insulation material, obtaining a binder calibration curve showing a relationship between particle size and compressibility of a binder using sets of particle size and compressibility of the binder, measuring particle sizes of the insulation material and the binder loaded, determining an optimal mixture weight ratio with reference to the curves so that compressibility of mixture powder of the insulation material and the binder equals a set compressibility based on the measured particle sizes, mixing the insulation material and the binder at the determined mixture weight ratio to form mixture powder, loading the mixture powder into a powder dispenser, and adding a solvent to the mixture powder.