A single-use bioreactor is provided. The single-use bioreactor may include a bioprocess container, a shell, at least one agitator, at least one sparger, at least one gas filter inlet port for the sparger(s) and headspace overlay, at least one fill port, at least one harvest port, at least one sample port, and at least one probe. In examples, at least one controller may monitor and control one or more parameters associated with the single-use bioreactor A method to cultivate and propagate mammalian cells is also provided. The method may include cultivating under suitable conditions and in a suitable culture medium in a first single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a second single-use bioreactor, transferring the medium containing the cells obtained by propagation from the at least one mammalian cell is into a third single-use bioreactor, and cultivating the cells in the third bioreactor.

This application relates to a method of treating wastewater wherein an oxygen infusion system is used to supersaturate wastewater before aerobic biological processes, wherein oxygen is transferred to the wastewater free of oxygen bubbles and achieves a reduction in power demand for the aeration process of wastewater.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.

A double shaft paddle mixer, including a barrel chamber, and a first paddle screw and a second paddle screw. The first paddle screw is provided with first paddles and the second paddle screw is provided with second paddles. The barrel chamber includes a crushing zone and a mixing zone. The crushing zone is partly separated from the mixing zone by a partition member. A first inlet is connected to the crushing zone and a second inlet is connected to the mixing zone and an outlet connected to the mixing zone. The double shaft paddle mixer can be part of an arrangement and part of a method for producing paste.

A mixing aerator is disclosed that includes a housing defining a chamber having a bottom end and a top end, the housing having at least one inlet and at least one outlet; and a longitudinally extending diffuser disposed within the chamber and configured to deliver air bubbles into the chamber when the chamber is filled with liquid. The diffuser includes (a) a tubular elastomeric membrane having a plurality of perforations and, within the tubular elastomeric membrane, (b) an air pipe having a plurality of openings, the openings being larger and fewer than the perforations, and the tubular membrane having upper and lower ends that are sealed against an outer surface of the air pipe.

An air diffuser includes: a bottom panel provided in a horizontal direction in a tank in which water is filled; an air diffusion body installed to cover the bottom panel from above; and air diffusion holes arranged to penetrate through the air diffusion body, gas fed to a gap between the bottom panel and the air diffusion body is discharged into water through the air diffusion holes, and an air diffusion region of the air diffusion body where the air diffusion holes are arranged has a width equal to or larger than 10 mm and smaller than 120 mm.

An apparatus for providing a liquid component as a first starting component of a bone cement dough from two starting components, comprising a receptacle in which a tank containing the liquid component can be stored, an opening means for opening the tank, a reservoir, fluidically connected to the receptacle, for receiving the liquid component from the tank, at least one port for fluidically connecting the apparatus to a syringe in which a powder component can be stored as a second starting component of the bone cement dough, and at least one conducting means which fluidically connects the reservoir and the at least one port to one another.

A mixing blade comprises a first blade, at least one second blade. The first blade is spirally formed around a first shaft. The at least one second blade is twisted around a second axis parallel to the first shaft. The second blade is provided between facing blade portions in the first blade. The facing blade portions faces each other in a direction of the first shaft.

The disclosure herein relates to a microfluidic emulsification device capable of being injection molded. The device may be used for digital droplet polymerase chain reaction (ddPCR). The emulsification device comprises: (a) a cylindrical outer part (4) with two open ends; (b) a cylindrical inner part (1) with a solid bottom and having a circumference sufficient to allow the inner part to be nested within the outer part of the emulsification device, wherein the inner part and the outer part are capable of sliding freely; (c) at least one groove on an interior surface of the outer part or on an exterior surface of the inner part, the groove having a height greater than a gap between the outer part and the inner part when nested; (d) at least one hole (3) in the inner part adjacent to the solid bottom; (e) a radial distribution channel (2) on the interior surface of the outer part or on the exterior surface of the inner part; and (f) a radial nozzle channel at the base of the interior surface of the outer part or at the base of the exterior surface of the inner part.

A stirrer is provided that can more efficiently achieve shearing applied, by an action of an intermittent jet flow, to a fluid to be processed. The stirrer concentrically includes a rotor including a blade, a partition wall, and a screen, wherein: the screen includes a plurality of slits in a circumferential direction thereof and screen members located between the adjacent slit; by rotating at least the rotor of the two components, the fluid to be processed is discharged from the inside to the outside of the screen as the intermittent jet flow through the slit of the screen; the screen has a cylindrical shape having a circular cross section; an opening of the slit provided on the inner wall surface of the screen is used as an inflow opening; openings of the plurality of slits provided on the outer wall surface of the screen are used as outflow openings; and the width of the outflow openings in the circumferential direction is set to be smaller than the width of the inflow opening in the circumferential direction.