Bioreactors are provided that include a vessel and a jet mixer disposed in the vessel. Methods that utilize the bioreactors are provided, involving placing a microorganism or cells and a fluid medium in the bioreactor.

Biosolids conversion systems and methods are provided. Wastewater may be received and separated into a separated water component and a dewatered solids component on-site at or near a wastewater source. The separated water components may be treated on-site using an ancillary water treatment process. The dewatered solids may be converted to a biosolid with aid of one or more oxidizers and a blending chamber. The dewatered solids may be converted to a biosolid on-site at or near a wastewater source or may be converted to a biosolid off-site at a central solids processing facility.

A manufacturing method for a granulated body includes supplying powder agitated by a dry agitator to a wet agitator, and agitating the powder supplied from the dry agitator with a liquid component in the wet agitator by rotating blades so as to form a granulated body. The blades are rotated when the powder is supplied to the wet agitator from the dry agitator. The dry agitator agitates the powder in a dry state, and the wet agitator is positioned perpendicularly below the dry agitator. The wet agitator includes an agitation chamber and the blades rotating around a center axis orthogonal to a direction in which the powder is supplied.

The invention relates to a method for preparing emulsions.

In order to create a new method for preparing emulsions, in which homogenous oil droplets as small as possible can be generated with an energy input as low as possible, it is proposed in the scope of the invention, that at least two liquid streams of liquids that cannot be intermixed with one another are pumped through separate openings with defined diameters, in order to achieve flow velocity of the liquid streams of more than 10 m/sec., and in that the liquid streams collide at a collision point in a space, wherein the resulting emulsion is discharged from the space through an outlet.

By the collision of the liquid streams with high flow velocities, in which a plate-shaped collision plate is formed in the collision point, a homogenous emulsion having an oil droplet size of less than 1 m is achieved due to the kinetic energy, which is accordingly very stable as well. No further energy input, such as shear forces, is required to that end.

In one example, a group of interchangeable supply modules to hold powdered build material for additive manufacturing. Each supply module in the group includes an exterior sized and shaped to fit into a mixer; an interior defining a non-circular mixing chamber, and an outlet through which powdered build material may leave the mixing chamber.

A solvent mixing system includes a mixing tee, a centrifugal mixing path, and a low frequency blending mixer. The mixing tee has at least two solvent input ports and a solvent output port in fluid communication with one another. The centrifugal mixing path has a mixing path inlet in fluid communication with the solvent output port of the mixing tee. The centrifugal mixing path includes at least one coiled segment between the mixing path inlet and a mixing path outlet. The low frequency blending mixer is in fluid communication with the outlet of the centrifugal mixing path.

The present invention is based in part on the discovery of significant improvements to cell culture systems and methods of generating organoids. The system of the invention provides a novel spinning bioreactor platform for higher-throughput 3D culturing of stem cells (e.g. human induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs)). The system can be widely used as a standard platform to generate stem cell-derived human organoids for any tissue and for high-throughput drug screenings, toxicity testing, and modeling normal human organ development and diseases.

The present invention relates to a method of making a composition, the composition comprising a cross-linked polymer and water, comprising at least the following steps: a) providing a first mixture, comprising at least one polymer and an aqueous alkaline solution; b) generating a gel by cross-linking the at least one polymer with a cross-linking agent; c) neutralizing the generated gel by using a neutralization solution; d) optionally adding a second mixture, comprising a cross-linked and/or non-cross-linked polymer and water, to the neutralized gel obtained in step c); e) dialyzing the gel obtained in step d) or dialyzing the gel obtained in step c) and optionally subsequent addition of the second mixture, comprising a cross-linked and/or non-cross-linked polymer and water; and f) filling the dialyzed gel obtained in step e) into a container, wherein at least two or three or four or five or all of steps a) to f) are automated.

A dynamic mixer (40) comprising a rotatable structure (10, 111, 11, 1, 2, 3, 4, 5) having a cylindrical base (14) having a connector disposed at one end, an opposing thinned end, flights of 3 to 6 blades (11, 12, 151, 61), separated by a notches (13, 18) the blades (11, 12, 151, 61) have an inlet face facing the connector end, an outlet (30) face facing the thinned end, a leading edge (21) facing the direction of rotation, a trailing edge (20) opposite the leading edge (21), a standard leading face (154, 65) which tapers from the leading edge (21) to the outlet (30) face and a standard trailing face (166) which tapers from the trailing edge (20) to the inlet face, or a reverse leading face (154, 65) which tapers from the leading edge (21) to the inlet face and a reverse trailing face (155, 66) which tapers from the trailing edge (20) to the outlet (30) face, the notches (13, 18) are offset from one another and the article is adapted for use in a dynamic mixer (40) to mix viscous material when rotated in the mixer (40). An article comprising the mixer (40), a shell (25, 28, 29, 31) about the mixer (40) and an endplate (33) that defines material inlets and seals the inlet end (15, 27) of the mixer (40).

A method is provided for monitoring a flow behavior of mixed components without requiring additional instrumentation or sampling. The method is carried out by determining ratios of the power required to rotate a mixing impeller at different rotational speeds and then comparing the ratios. Characteristics about the mixed components are determined based on differences between the ratios.