The present invention is based in part on the discovery of significant improvements to cell culture systems and methods of generating organoids. The system of the invention provides a novel spinning bioreactor platform for higher-throughput 3D culturing of stem cells (e.g., human induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs)). The system can be widely used as a standard platform to generate stem cell-derived human organoids for any tissue and for high-throughput drug screenings, toxicity testing, and modeling normal human organ development and diseases.

A method is provided for monitoring a flow behavior of mixed components without requiring additional instrumentation or sampling. The method is carried out by determining ratios of the power required to rotate a mixing impeller at different rotational speeds and then comparing the ratios. Characteristics about the mixed components are determined based on differences between the ratios.

In an embodiment, a reactor for carrying out a melt transesterification reaction at a reactor temperature of 160 to 300 C. and a reactor pressure of 5 to 200 mbar, comprises a cylindrical tank comprising a top, a side, and a bottom, wherein the bottom is convex, extending away from the top; a stirring shaft disposed within the cylindrical tank along an axis thereof so that it is rotatable from outside of the cylindrical tank; an impeller extending from the stirring shaft in the cylindrical tank and comprising a plurality of blades; a reactant solution inlet; a reaction solution outlet; and an externally located heat exchanger in fluid communication with the cylindrical tank via a recirculation stream and a heated stream. The reactor can be used for the polymerization of a polycarbonate oligomer.

An apparatus for enhancing phase contact and chemical reactions is provided. The apparatus comprises at least one first high-turbulence mixing stage and at least one second high-shear-stress and high-cavitation stage. The stages are adapted to cause an increase in the relative sliding speeds of the phases involved in a multiphase flow passing through the stages.

A cavitation device and method for using the same is useful for cavitationally treating fluids by generation of hydrodynamic cavitation in the fluid followed by the subsequent collapse of cavitation bubbles. The passage of fluid through slot openings in a cylindrical insert mounted in a housing provides fluid jets in an annular cavity to induce hydrodynamic cavitation of the fluid. Fluid is discharged from the annular cavity into a downstream portion of the housing to collapse cavitation bubbles under static pressure.

A method of mixing using a tubular reactor wherein process material continuously passes through the tubular reactor which is operating at predetermined reaction conditions. The tubular reactor is rotated through reciprocating arcs about the longitudinal axis of the tube as the process material passes therethrough. Static and/or dynamic mixers or agitators may be provided within the tubular reactor.

Provided is a mixing apparatus. The mixing apparatus comprises a housing defining a primary chamber, an inlet for receiving material into the mixing apparatus, as well as an outlet for discharging material from the mixing apparatus. The housing provides within the primary chamber a plurality of rotating shafts, each rotating shaft having a plurality of flailing fixtures associated therewith.

A horizontal mixer for mixing bulk material, such as agricultural material, is provided. The horizontal mixer includes a mixing container for accommodating bulk agricultural material to be mixed. The mixing container includes side walls connected at their bottom edges by a trough; a front wall spanning a front side of the container; a rear wall spanning a rear side of the container; and an exit door through which mixed agricultural material can exit. Further, one or more bottom augers situated longitudinally in the trough, wherein at least one of the one or more bottom augers is a ribbon auger; and one or more top augers situated longitudinally in the mixing container stacked above the one or more bottom ribbon augers. The trough has a shape to accommodate the one or more bottom ribbon augers.

A reciprocating motion disk for mixing wastewater in a tank of a treatment plan is optimized for geometry, along with cycling speed and stroke length, to cause effective mixing velocity throughout the tank.

A cell culture system and method of generating organoids is provided. The system includes a multiwell culture plate having shafts for mixing culture media within wells of the culture plate. The multiwell culture plate includes a base substrate having a plurality of culture wells, and a shaft operably associated with each culture well, each shaft being configured to mix media present in each culture well and having a gear adapted to operably associate with a gear on a shaft associated with an adjacent culture well. The system further includes a motor having a drive shaft in operable communication with the shaft gears of the multiwell culture plate. The system can be widely used as a standard platform to generate stem cell-derived human organoids for any tissue and for high-throughput drug screenings, toxicity testing, and modeling normal human organ development and diseases.