The present invention provides a method for the production of whey proteins in a single step process using combination of chromatography and membrane filtration technique, comprising treating cotton cloth with a mixture of chlorosulphonic acid and chloroform and then subsequently treating it with chloroform, dilute NaOH, glycine and water to recover modified cotton cloth as the product, thereafter fixing product in a membrane filtration device equipped with modified flow pattern and then equilibrating it with equilibration buffer, followed by loading of whey for adsorption of protein on the product and washing of the product with equilibration buffer, thereafter elution of adsorbed proteins with elution buffer, and then regeneration of the product by treating it with dilute HCl and water to reuse the product.

In some embodiments, the present invention provides a method of purifying a protein of interest with a reduced level of aggregation formation in cation exchange (CEX) chromatography, comprising: (a) providing a mixture comprising the protein of interest and one or more contaminants; (b) loading the mixture onto a CEX resin coupled with arginine; and (c) eluting the protein of interest from the resin, thereby purifying the protein of interest with a reduced level of aggregation formation in CEX chromatography.

In some embodiments, the present invention provides a method of purifying a protein of interest with a reduced level of aggregation formation in cation exchange (CEX) chromatography, comprising: (a) providing a mixture comprising the protein of interest and one or more contaminants; (b) loading the mixture onto a CEX resin coupled with arginine; and (c) eluting the protein of interest from the resin, thereby purifying the protein of interest with a reduced level of aggregation formation in CEX chromatography.

In some embodiments, the present invention provides a method of purifying a protein of interest with a reduced level of aggregation formation in cation exchange (CEX) chromatography, comprising: (a) providing a mixture comprising the protein of interest and one or more contaminants; (b) loading the mixture onto a CEX resin coupled with arginine; and (c) eluting the protein of interest from the resin, thereby purifying the protein of interest with a reduced level of aggregation formation in CEX chromatography.

In some embodiments, the present invention provides a method of purifying a protein of interest with a reduced level of aggregation formation in cation exchange (CEX) chromatography, comprising: (a) providing a mixture comprising the protein of interest and one or more contaminants; (b) loading the mixture onto a CEX resin coupled with arginine; and (c) eluting the protein of interest from the resin, thereby purifying the protein of interest with a reduced level of aggregation formation in CEX chromatography.

The present invention provides a method for the production of whey proteins in a single step process using combination of chromatography and membrane filtration technique, comprising treating cotton cloth with a mixture of chlorosulphonic acid and chloroform and then subsequently treating it with chloroform, dilute NaOH, glycine and water to recover modified cotton cloth as the product, thereafter fixing product in a membrane filtration device equipped with modified flow pattern and then equilibrating it with equilibration buffer, followed by loading of whey for adsorption of protein on the product and washing of the product with equilibration buffer, thereafter elution of adsorbed proteins with elution buffer, and then regeneration of the product by treating it with dilute HCl and water to reuse the product.

The present disclosure relates to lignocellulosic materials comprising phosphorylated lignocellulosic fibers having an ionic charge in water of about 4000 to about 7000 mmoles/kg, and processes for the preparation thereof. The process comprises reacting lignocellulosic fibers of a lignocellulosic material with a phosphate ester in the presence of urea. The present disclosure further relates to compositions comprising a phosphate ester and at least one of a defoamer and a viscosity reducer or at least one of a C1-C12 alcohol and an ester of a carboxylic acid.

Provided is a methodology to improve soil performance by dispersing stabilized low C:N non-highly polymerized porous lignocellulose catalytic that improves porosity, carbon capture, and microbial activities.

The invention relates to processing methods to produce Bee Product Extracts produced from a Bee Product such as honey, pollen, waxes, or royal jelly; and in particular a Bee Product Extract produced from manuka honey; their uses, methods of preparation and methods of their detection and analysis. The Bee Product Extract of the invention contains phenolics, minerals and proteins coordinated in a complex that undergoes photo-Fenton chemistry process to generate high energy short lived radicals, including Hydroxyl radicals and superoxide radicals. The formulated Bee Product Extract can provide a topical form to be used as a spray, mist or serum for the treatment of a wide range of human and animal diseases and to maintain health and well-being.

The invention relates to processing methods to produce Bee Product Extracts produced from a Bee Product such as honey, pollen, waxes, or royal jelly; and in particular a Bee Product Extract produced from manuka honey; their uses, methods of preparation and methods of their detection and analysis. The Bee Product Extract of the invention contains phenolics, minerals and proteins coordinated in a complex that undergoes photo-Fenton chemistry process to generate high energy short lived radicals, including Hydroxyl radicals and superoxide radicals. The formulated Bee Product Extract can provide a topical form to be used as a spray, mist or serum for the treatment of a wide range of human and animal diseases and to maintain health and well-being.