A method and apparatus for the manipulation for an identification purpose of a microcarrier. The method comprising the steps of: (a) an identification purpose step of the microcarrier; and (b) a positioning and orientation step prior to or during the identification purpose step. The apparatus comprising means for identification purposes such as a microscope or labelling means such as a high spatial resolution light source, and means for the positioning and orientation of the microcarriers.

The present invention provides improved methods, facilities and systems for parallel processing of biological cellular samples in an efficient and scalable manner. The invention enables parallel processing of biological cellular samples, such as patient samples, in a space and time efficient fashion. The methods, facilities and systems of the invention find particular utility in processing patient samples for use in cell therapy.

A parallelized chain-synthesizing technique includes an array of wells, each well in the array providing a location where a specific arbitrary sequence for polymeric chains can be grown. An optical addressing system selectively delivers light to the wells to mediate or control reactions in the wells.

The invention features methods of making devices, or platens, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

Positioning structures in at least one indentation present on a or in a support, wherein said indentation is an indentation having a diameter in the nanometer range, makes it possible to position the structure in the indentation substantially centrally with defined orientation. A support having at least one indentation, wherein the indentation has a size in the nanometer range, includes a predetermined structure which is arranged substantially centrally within said indentation and which optionally has a functional unit diametrically opposite to the side pointing to the bottom surface. The arrangement is especially suitable for single molecule analysis and, here especially, for single molecule sequencing and other high-throughput methods.

Provided is a method of synthesis comprising: (I) providing separate reaction sequences to TABs; (II) utilizing reaction vessels configured to react a separate combinatorial building block with a moiety on a surface of a TAB; and (III) operating one or more TAB sorters comprising a TAB reader, a sorting tree comprising valves or switches and sorting nodes, and a monitor configured to detect TAB location, wherein the operating comprises serially conducting: (a) reacting distinct combinatorial building blocks in the reaction chambers with surfaces of TABs distributed in the reaction chambers; (b) operating a controller to operate the TAB sorters to segregate the TABs to allocations appropriate for the next assigned reaction, the operating including recycling TABs with ambiguous identity back through the sorter; and (c) repeating steps (a) and (b) as needed to complete 30% or more of the assigned sequences.

Disclosed are devices, compositions, and methods useful for assessing properties of compounds and molecules, such a binding, kinetic, and enzymatic properties, simultaneously for multiple compounds or molecules and/or under multiple conditions, efficiently, rapidly, and combinations of these. By using certain features alone or together in the save device or assay, the disclosed devices, compositions, and methods provide improvements over, and solve problems present in, prior assay devices and methods.

Described herein are techniques that improve the collection and readout of charge carriers in an integrated circuit. Some aspects of the present disclosure relate to integrated circuits having pixels with a plurality of charge storage regions. Some aspects of the present disclosure relate to integrated circuits configured to substantially simultaneously collect and read out charge carriers, at least in part. Some aspects of the present disclosure relate to integrated circuits having a plurality of pixels configured to transfer charge carriers between charge storage regions within each pixel substantially at the same time. Some aspects of the present disclosure relate to integrated circuits having three or more sequentially coupled charge storage regions. Some aspects of the present disclosure relate to integrated circuits capable of increased charge transfer rates. Some aspects of the present disclosure relate to techniques for manufacturing and operating integrated circuits according to the other techniques described herein.

De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.