The present invention provides for a fully integrated microfluidic system capable of producing single-dose amounts of biotherapeutics at the point-of-care wherein protein production, purification and product harvest are all integrated as a single microfluidic device which is portable and capable of continuous-flow production of biotherapeutics at the microscale using a cell-free reaction system.

In some embodiments, a variety of devices and methods for conducting microfluidic analyses are utilized herein, including devices that can be utilized to conduct thermal cycling reactions such as nucleic acid amplification reactions. The devices include elastomeric components; in some instances, much or all of the device is composed of elastomeric material. Amplification products (amplicons) can be detected and distinguished (whether isolated in a reaction chamber or at any subsequent time) using routine methods for detecting nucleic acids. An example of a detection method includes hybridization to arrays of immobilized oligo or polynucleotides.

A method for synthesizing a nucleic acid includes synthesizing one or more nucleic acid fragments on a substrate. The synthesized one or more nucleic acid fragments may be amplified on the substrate. The method also includes sequencing the synthesized or amplified one or more nucleic acid fragments on the substrate. The sequencing may provide feedback to designs of the one or more nucleic acid fragments. The method further includes harvesting the synthesized or amplified one or more nucleic acid fragments based on sequencing. The synthesized or amplified one or more nucleic acid fragments may be assembled to generate a target nucleic acid.

Apparatus and methods are disclosed for electrically active combinatorial-chemical (EACC) chips for biochemical analyte detection. An apparatus includes a substrate that has an array of regions defining multiple cells, wherein each of the cells includes a reaction cavity that contains multiple functional binding groups. A method of detecting an analyte providing the reaction cavity between a source and a drain or a pair of electrodes, applying a voltage and monitoring a parameter indicative of an analyte characteristic. A process of fabricating an EACC include bonding an analyte to the multiple functional binding groups of each reaction cavity, and forming an analyte sensing structure including the substrate.

A method for synthesizing a nucleic acid includes synthesizing one or more nucleic acid fragments on a substrate. The synthesized one or more nucleic acid fragments may be amplified on the substrate. The method also includes sequencing the synthesized or amplified one or more nucleic acid fragments on the substrate. The sequencing may provide feedback to designs of the one or more nucleic acid fragments. The method further includes harvesting the synthesized or amplified one or more nucleic acid fragments based on sequencing. The synthesized or amplified one or more nucleic acid fragments may be assembled to generate a target nucleic acid.

Technologies for rapid equilibration for water isotope analysis are disclosed. In at least one illustrative embodiment, a vaporizer may include an injection block that defines a chamber and a septum positioned over an inlet of the chamber to seal the chamber. The chamber may be configured to be fluidly coupled to a pump to develop a vacuum within the chamber, and the septum may be configured to receive a needle that is inserted into the chamber. A thermally conductive wool may be positioned within the chamber and may be configured to receive a tip of the needle.

The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in loading of polymerase enzyme complexes into a predetermined number of reaction sites, including nanoscale wells.

The invention relates to an apparatus for analyzing reaction systems with a liquid phase (13) and a gas phase (15), the apparatus (1) comprising at least two tank reactors (3), a common feed line (5), a common drain line (25) for the liquid phase and a common drain line (21) for the gas phase, each tank reactor (3) being connected to the common feed line (5) by a supply line (7), to the common drain line (25) for the liquid phase by a liquid withdrawal line (27) and to the common drain line (21) for the gas phase by a gas withdrawal line (23), wherein the pressure in each tank reactor (3) is controlled by one of: (a) a pressure control (31) in the common feed line (5); (b) a pressure line (29) which is connected to the gas space of each tank reactor (3); (c) a pressure control (31) in the common drain line (21) for the gas phase and a flow restrictor (33) in the common drain line (25) for the liquid phase or a pressure control (31) in the common drain line (25) for the liquid phase and a flow restrictor (33) in the common drain line (21) for the gas phase; or (d) a pressure line (29) which enters into the common drain line (25) for the liquid phase or into the common drain line (21) for the gas phase.

The invention further relates to a process for analyzing reaction systems in such an apparatus.

The invention consists of an assembly of a light (e.g., UV, visible, IR) source, a reaction vial holder and a photochemistry device that allows for conducting arrays of photochemical reaction conditions at room temperature with magnetic stirring. The photochemistry assembly is compatible with multiple reaction vial size holder.

An apparatus suitable for investigating solid catalysts and processes in which discontinuous fluid streams arise, the apparatus including: a reactant fluid supply point; a reaction space; at least one fluid mixing space; at least one throttle element; at least one pressure control valve; and at least one analyzer. An outlet side of the reaction space is operatively connected to the fluid mixing space via a connecting line and a substream line. The fluid mixing space is connected to the throttle element. The throttle element is operatively connected to the analyzer and an outlet line. The connecting line is operatively connected to the pressure control valve and an exit air line. The pressure control valve is arranged either downstream or upstream of the substream line. When the pressure control valve is upstream of the substream line, the outlet line is provided with a second pressure control valve and a pump.