A cell extraction method includes providing a mixed-cell sample having both target cells and non-target cells slurried in a trailing-electrolyte solution. A leading electrolyte solution is provided to contact the trailing electrolyte solution via a capillary and a pressure control device. Focusing of the target cells at an interface between the leading- and trailing-electrolyte solutions occurs by applying pressure to the capillary and concurrently applying an electric potential via electrodes. The target cells are extracted into the capillary by applying a negative pressure and transferred to a receptacle. A cell extraction system for use with a gradient elution isotachophoresis method includes a trailing electrolyte solution adapted to migrate slower than the target cells and a leading electrolyte solution adapted to migrate faster than the target cells. A mixed-cell sample containing the target and non-target cells is slurried in the trailing electrolyte solution prior to cell extraction.

A new simple transfection method using an integrated electrowetting nano-injector (INENI) with controlled dosage delivery and high transfection efficiency is disclosed. The volume of delivery can be controlled via voltage application to an inner and outer electrode integrated into a nano-pipette. With higher voltages, more liquid enters the INENI and with lower voltages liquid is expelled. This method can be used to deliver plasmid DNA directly into the nuclei of cells. The INENI requires only the use of a single probe since both electrodes are integrated into the same nano-pipette. Hence, more space is available, and ergo the INENI offers a simplistic means for direct injection of metered amounts of exogenous material into the confines of a cell cytoplasm and/or nucleus while retaining full cell viability.

A handheld solids dispenser. The dispenser includes a housing defining an inlet passage and an outlet passage. A delivery mechanism is supported within the housing and is positioned between the inlet passage and the outlet passage. Actuation of the delivery mechanism causes the delivery mechanism to transport a desired amount of solids material from the inlet passage to the outlet passage. The delivery member may be in the form of a plunger slidably positioned within the housing. The delivery mechanism may be in the form of a screw conveyor driven by a drive motor.

An assay cartridge has a base member (26) that defines at least two wells (30, 32, 34, 36, 38), a pipette (108, 110) positionable in at least one of the wells and a cap member (86) arranged to carry the pipette. The cap member can be releasably fastened to the base member. An extension member (28) defines at least one further well (40, 42, 44) and can be fastened to the base member such that the pipette is then positionable in at least one of the wells of the base and in the further well of the extension member.

Systems and methods for nesting refill filtered pipette tips to reduce storage space and plastic waste are provided herein. Embodiments include a lower filtered refill wafer comprising a lower plurality of refill filtered pipette tips in a nested filtered pipette tip rack, the lower filtered refill wafer comprising the lower plurality of refill filtered pipette tips having holes between top portions of the lower plurality of refill filtered pipette tips. Various embodiments further include an upper filtered refill wafer comprising an upper plurality of refill filtered pipette tips nested into the holes between the top portions of the lower plurality of refill filtered pipette tips, the nesting of the upper filtered refill wafer thereby reducing storage space for the nested filtered pipette tip rack compared with storage space for a filtered pipette tip rack that is not nested.

A capillary of the present disclosure has a tubular shape, both ends of which are a first end and a second end in a length direction and are open, an inner surface of which has water repellency and has a portion where a degree of the water repellency changes. A pipette of the present disclosure includes the capillary and a pipette main body to which the capillary 10 is attached, and the pipette main body has a pressure chamber that is deformable and connected to the capillary.

A cell extraction method includes providing a mixed-cell sample having both target cells and non-target cells slurried in a trailing-electrolyte solution. A leading electrolyte solution is provided to contact the trailing electrolyte solution via a capillary and a pressure control device. Focusing of the target cells at an interface between the leading- and trailing-electrolyte solutions occurs by applying pressure to the capillary and concurrently applying an electric potential via electrodes. The target cells are extracted into the capillary by applying a negative pressure and transferred to a receptacle. A cell extraction system for use with a gradient elution isotachophoresis method includes a trailing electrolyte solution adapted to migrate slower than the target cells and a leading electrolyte solution adapted to migrate faster than the target cells. A mixed-cell sample containing the target and non-target cells is slurried in the trailing electrolyte solution prior to cell extraction.

A blood sample collection and/or storage device includes a two-piece housing that encompasses a port at which a fingertip blood sample is collected. After the sample is taken, the two-piece housing is moved to a closed position to protect the sample for storage and optionally process the sample within the housing. The housing may also be opened to access the stored sample for further processing.

A microfluidic reagent card for medical testing and assaying is disclosed. The microfluidic reagent card comprises a card body (10) and a plurality of micropipes, the plurality of micropipes is fixed onto the card body (10). The plurality of micropipes is arranged radially. Each of the micropipe is configured to have a sample inlet end (1) and a closed end (2), and a detection liquid layer (3) and a separation medium layer (4) are arranged inside each micropipe in sequence from the sample inlet end (1) to the closed end (2). The micropipe has a diameter of 0.1-1.0 mm. As the micropipe is configured to have a small diameter, the fluidity of liquid and gel decreases inside the micropipe, and the liquid interface will not flow even when the micropipe is placed in a horizontal direction.

Devices, kits, and methods related to embodiments of an improved liquid test sample injection device for use in diagnostic assays and for the collection of liquid waste produced from such diagnostic assays.