Disclosed are a micro-column gel card, and a sample adding mechanism and method. The micro-column gel card includes a fixing plate and a plurality of tubular columns (3) arranged and fixed through the fixing plate. The tubular columns (3) are fixed to two sides of the fixing plate respectively, and the tubular columns (3) located on the two sides of the fixing plate are arranged in a staggered manner. Each tubular column (3) includes a sample adding cavity (301), a reaction cavity (302), and a gel column (303). The gel column (303) is configured to load a gel reagent. A central axis of the sample adding cavity (301) and a central axis of the gel column (303) do not coincide. The tubular columns (3) are designed in a double-row staggered manner.

A method of recovering the contents from a microcapillary array having a plurality of wells each with mutually opposed first and second openings. The method comprises: a) identifying a target well of the array for recovery from among a plurality of wells of the array; b) bringing first and second recovery ducts into contact with the array in alignment respectively with the first and second openings of the identified target well to effect fluid communication between those ducts and that well through the respective first and second openings; and c) by flowing fluid from the first recovery duct through the identified target well and into the second recovery duct, conveying contents of that well into the second recovery duct.

An autoclaving microplate washing system for cells and non-adhering three-dimensional (3D) cell cultures includes one or more pumps for controlling the dispensing of washing fluid and the evacuation of fluid from microwells to gently wash the cells. A method of controlling the autoclaving microplate washing system includes controlling the one or more pumps for dispensing and evacuation.