An apparatus suitable for single molecule sequencing. The apparatus includes at least one nanowell, a plurality of nucleic acid immobilization moieties, and a plurality of types of nucleic acid fragments. The nanowell has an observation zone. The nucleic acid immobilization moieties are disposed in or proximate to the observation zone. The nucleic acid fragments are immobilized to the nucleic acid immobilization moieties, respectively. At least one polymerase is disposed in the observation zone. A method of sequencing nucleic acid molecules using the above-mentioned apparatus is provided.

A high-throughput and rapid nucleic acids detection method based on capillary microarrays comprises the steps that firstly, microarray containing a number of hydrophilic and vertical micro-channels is fabricated by capillary assembling, casting and machining, and the outer surface of the capillary array is coated with super-hydrophobic Ultra-Ever Dry paint; secondly, different primer sets are individually loaded into the micro-channels and air-dried to adhere them on the inner surface, and then the microarray is anchored into a reaction tube; thirdly, the reaction mixture is introduced into every microchannel at once through capillary force by a special designed sample-loading adaptor, and then the amplification reaction is performed in the temperature control device; and finally, the fluorescence can either be measured continually during the reaction for real-time detection or be recorded once in the end for endpoint detection. Moreover, the products can also be recovered for other use later.

A method and system are provided for extracting a target analyte from a sample using acoustic ejection technology. The method involves applying focused acoustic energy to a fluid reservoir housing a fluid composition that contains a target analyte and comprises an upper region and a lower region, where the concentration of the target analyte in the upper region differs from that in the lower region. The focused acoustic energy is applied in a manner that is effective to result in the ejection of a fluid droplet from the fluid composition into a droplet receiver, wherein the concentration of the analyte in the droplet corresponds to either the concentration of the analyte in the upper region or the concentration of the analyte in the lower region, and wherein the concentration of the analyte is substantially uniform throughout the droplet. The fluid composition may comprise an ionic liquid, used in the extraction of ionic target analytes. Related methods and an acoustic extraction system are also provided.

Provided herein is a system comprising: a) spatial sampler system configured to collect and transmit one or a plurality of cells or nuclei from a tissue specimen, the system comprising: i) a lower carrier having an array of conduits passing therethrough, each conduit comprising an opening on a first side and communicating with an opening on a second side, wherein the opening on the second side either (1) terminates in a capillary or (2) opens onto a well of a multiwell plate; ii) positioned, above the lower carrier, a perforated specimen holder configured to support a frozen tissue specimen, wherein the specimen holder comprises a plurality of perforations having a size sufficient to permit the passage of single cells or nuclei; and iii) positioned, above the specimen holder, a multifunctional head comprising an upper array of upper conduits, optionally, each aligned with a conduit opening of the lower carrier.

Aspects of the present disclosure relate to a method and/or a device for carrying out chemical, biochemical and/or immunochemical analyses of liquid samples, which are present in a sample store of an automatic analyzer, with the aid of liquid reagents which are present in at least one reagent store of the analyzer. In one example embodiment, the automatic analyzer includes cuvettes, a first pipettor, a device with an optical measurement unit, a device for heterogenous immunoassays, a cuvette washing unit, a needle washing unit, a temperature control unit.

A sample storage device may include: a storage chamber; paired rail sections separated in separating direction and extending in an extending direction; an antenna array substrate extending between the rail sections and having antennas; paired slide sections separated from each other in the separating direction and disposed at a position opposite to the antenna array substrate with respect to the rail sections and, so as to be slidable in the extending direction relative to the rail sections, respectively; a support plate transparent to radio waves, extending between the slide sections; and a storage box containable a plurality of containers each holding a sample and a radio tag, the storage box to be set on the support plate such that radio waves can be transmitted between the radio tag and corresponding one of the plurality of antennas.

This invention discloses a design of microplates and microarrays to improve utility. The new design constructs a physical flux barrier that limits thermocapillary and other mass transfer contributions to the heterogeneous accumulation of reactant at the surface of a well or array address. The improved control of reactant delivery results in a much more uniform distribution of reactant across an address, thereby improving the accuracy of the measured response.

Systems and methods for optical power distribution within an integrated device, in a substantially uniform manner, to a large number of sample wells and/or other photonic elements. The integrated device and related instruments and systems may be used to analyze samples in parallel. The integrated device may include a grating coupler configured to receive light from an excitation source and optically couple with multiple waveguides configured to couple with sample wells. Vertical extents of optical modes of individual waveguides may be modulated to adjust confinement of light within the waveguides. This modulation may enable more uniform distribution of excitation light to the sample wells, improve excitation efficiency, and prevent overpower on regions of the integrated device.

Various examples are provided for disposable medical sensors that can be used for detection of SARS-CoV-2 antigen, cardiac troponin I, or other biosensing applications. In one example, a medical sensing system includes single-use disposable test strip comprising a functionalized sensing area configured to detect SARS-CoV-2 antigen and a portable sensing and readout device including pulse generation circuitry that can generate synchronized gate and drain pulses for detection and quantification of SARS-CoV-2 antigen in biological samples. In another example, a method includes providing a saliva sample to a functionalized sensing area configured to detect SARS-CoV-2 antigen, generating synchronized gate and drain pulses for a transistor, the gate pulse provided via electrodes of the functionalized sensing area, and sensing an output of the transistor that is a function of a concentration of SARS-CoV-2 antigen in the sample.

A method and system are provided for extracting a target analyte from a sample using acoustic ejection technology. The method involves applying focused acoustic energy to a fluid reservoir housing a fluid composition that contains a target analyte and comprises an upper region and a lower region, where the concentration of the target analyte in the upper region differs from that in the lower region. The focused acoustic energy is applied in a manner that is effective to result in the ejection of a fluid droplet from from the fluid composition into a droplet receiver, wherein the concentration of the analyte in the droplet corresponds to either the concentration of the analyte in the upper region or the concentration of the analyte in the lower region, and wherein the concentration of the analyte is substantially uniform throughout the droplet. The fluid composition may comprise an ionic liquid, used in the extraction of ionic target analytes. Related methods and an acoustic extraction system are also provided.