Compositions of matter, methods, modules, and automated instruments may relate to synthesizing a library including an editing cassette including a different gRNA and donor DNA pair, amplifying the editing cassette in a partition separate from other editing cassettes in the library, adding nuclease to the partition, and adding lipofectamine to the editing cassette and nuclease to form a lipofectamine/nucleic acid/nuclease complex. A microcarrier coated in extracellular matrix or a cell adhesion molecule coating may be added to the lipofectamine/nucleic acid/nuclease complex. Cell growth material, the microcarrier, and mammalian cells may be transferred to a growth module in an automated closed cell editing instrument via a liquid handling system. The mammalian cells may be allowed to seed on the microcarrier. Conditions may be provided for the mammalian cells to take-up and be edited by a payload associated with the lipofectamine/nucleic acid/nuclease complex. The mammalian cells may be detached from the microcarrier.

Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. Additional analyte detection devices configured to operate an analyte detection chip to prepare a test sample and to detect an analyte related signal from the prepared test sample in the analyte detection chip are disclosed. The analyte detection chip may include a digital microfluidics (DMF) region and an analyte detection region which may overlap or may be spatially separated. The analyte detection device may be configured for detection of analyte by an optical or electrochemical means operably connected with an analyte detection chip inserted into the device.

A method for aligning sequences of droplets in streams of an emulsion comprising target droplets and tag droplets, a tag droplet comprising first and second tags. A target droplet is split into first and second target droplets and a tag droplet is split into first and second tag droplets. Each of the first and second tag droplets comprise the first and second tags. The first target droplet and first tag droplet are in a first stream of droplets, and the second target droplet and second tag droplet are in a second stream of droplets. The method detects the first tag droplets and first target droplets in the first stream and the second tag droplets and second target droplets in the second stream, determines a first sequence of droplets in the first stream and a second sequence of droplets in the second stream, and compares these to align the sequences.

An object separator may include a substrate, a fluid channel supported by the substrate, a pair of electrodes along the fluid channel to form a dielectrophoretic force to interact with an object entrained in a fluid, and an inertial pump supported by the substrate and positioned within the fluid channel to move the fluid along the fluid channel.

A microchamber array device having built-in reaction microchambers, in which the dilution ratio can be greatly increased at the same time as dramatically raising cell recovery efficiency, and an inspection object analysis method using said device are provided. This microchamber array device is provided with: a microchamber array 1 for cell capture by electrophoresis comprising an arrangement of a substrate 2, electrodes 3 and photoresists 4; and a reaction microchamber array 6 which is separated from the capture microchamber array 1, and which is formed from reaction microchamber 8 comprising micro channels 7 arranged so as to be opposite of the aforementioned microchamber array 1.

A driving method for a digital microfluidic chip, the digital microfluidic chip including a first electrode and a second electrode that are adjacent, the driving method including: applying a first driving signal to the first electrode and a second driving signal to the second electrode, wherein an applying period of the first driving signal and an applying period of the second driving signal are mutually staggered, and a total time length of the applying period of the first driving signal is less than a total time length of the applying period of the second driving signal.

Microfluidic devices in which electrokinetic mechanisms move droplets of a liquid or particles in a liquid are described. The devices include at least one electrode that is optically transparent and/or flexible.

A blood testing device for detecting hemolysis in a blood sample is described. The blood testing device comprises an housing for containing the blood sample. The housing has a treatment window and an optical zone formed therein. The blood testing device further includes an acoustic transducer positioned to selectively generate acoustic forces directed into the treatment window of the housing and a control unit for selectively actuating and deactuating the acoustic transducer to permit colorimetric analysis of plasma within the blood sample.

A pipette based on surface charges includes a pipette body. A plurality of support rods are provided below the pipette body. Connecting rods are provided at the lower ends of two adjacent support rods. A substrate is provided between the connecting rods. A porous SiO.sub.2 coating is provided on the substrate. A hydrophobic molecule layer is provided on the surface coating by the vapor deposition method. A superamphiphobic surface is formed on the substrate by the surface coating and the hydrophobic molecule layer. The substrate and the surface coating are made of dielectric materials. A circular limit plate is provided above the pipette body. The pipette body, the support rods, the connecting rods and the limit plate are integrally formed, and are electrically non-conductive. An electrically conductive sliding rod is movably provided within the pipette body and the limit plate.

Sub-micrometer bioparticles are separated by size in a microfluidic channel utilizing a ratchet migration mechanism. A structure within the microfluidic channel includes an array of micro-posts arranged in laterally shifted rows. Reservoirs are disposed at each end of the microfluidic channel. A biased AC potential is applied across the channel via electrodes immersed into fluid in each of the reservoirs to induce a non-uniform electric field through the microfluidic channel. The applied potential comprises a first waveform with a first frequency that induces electro-kinetic flow of sub-micrometer bioparticles in the microfluidic channel, and an intermittent superimposed second waveform with a higher frequency. The second waveform selectively induces a dielectrophoretic trapping force to selectively impart ratchet migration based on particle size for separating the sub-micrometer bioparticles by size in the microfluidic channel.