A method of preparing a Metal Organic Framework (MOF) with an acoustically-driven microfluidic platform, the method comprising: depositing a liquid comprising MOF precursors on a piezoelectric substrate of an acoustic microfluidic platform, the MOF precursors comprising a metal ion and an organic ligand, applying acoustic irradiation to the liquid to induce azimuthal liquid recirculation, which causes formation of the MOF within the liquid, and isolating the MOF.

A fluid device includes: a flow path through which a fluid flows; and an ultrasonic element configured to transmit an ultrasonic wave to the fluid to generate a standing wave in the fluid in the flow path along a first direction orthogonal to a flowing direction of the fluid. The ultrasonic element includes a vibrator having a fluid contact surface that comes into contact with the fluid, and a piezoelectric element that is provided at the vibrator and that is configured to flexurally vibrate the vibrator in a normal direction of the fluid contact surface. When a thickness of the vibrator in the normal direction is t, a sound velocity of a medium of the fluid is C, an average sound velocity of a longitudinal wave transmitted in the vibrator is C′, a dimension of the flow path in the first direction is L, and a mode order of the standing wave is n, the following expression is satisfied.

[00001]$t<\frac{C^{\prime }}{C}\times \frac{L}{2n}$

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

The present disclosure is drawn to temperature-cycling microfluidic devices. In one example, a temperature-cycling microfluidic device can include a driver chip having a top surface and a heat exchange substrate having a top surface coplanar with the top surface of the driver chip. A fluid chamber can be located on the top surface of the driver chip. A first and second microfluidic loop can have fluid driving ends and fluid outlet ends connected to the fluid chamber and can include portions thereof located on the top surface of the heat exchange substrate. A first and second fluid actuator can be on the driver chip. The first and second fluid actuators can be associated with the fluid driving ends of the first and second microfluidic loops, respectively, to circulate fluid through the first and second microfluidic loops.

A test device having a micro flow channel including a reaction part where a reactant that is reactive to a tested chemical dispersed in a tested fluid is fixed, and at least one actuator for actuating the tested fluid to move in at least one of two opposite sides of the micro flow channel so as to homogenize a density distribution of the tested chemical in the tested fluid. The tested fluid is sent in the micro flow channel a plurality of times.

This disclosure relates to a system for sorting sperm cells in a microfluidic chip. In particular, various features are incorporated into the system for aligning and orienting sperm in flow channels, as well as, for determining sperm orientation and measuring relative DNA content for analysis and/or sorting.

A microfluidic chip pump is provided. The microfluidic chip pump may include: a pump housing comprising a pump cavity, a moveable member arranged in the pump cavity, separating the pump cavity into a first chamber and a second chamber; and an actuator assembly configured to drive the moveable member between a first stable position and a second stable position, changing a volume of the first chamber and a volume of the second chamber. When the moveable member is at the first stable position, the first chamber may reach a minimum volume. When the moveable member is at the second stable position, the first chamber may reach a maximum volume. The microfluidic chip pump may be configured to expel a fixed volume of fluid from the second chamber each time the moveable member is driven from the first stable position to the second stable position.

A microfluidic chip system includes an input for receiving the biologic sample, and a first reading window for enabling a detection of the biologic material within the biologic sample. A first plurality of pathways is provided each for determining a treatment agent providing a best treatment efficacy for the predetermined biologic material. A first micro-pump is provided for pumping a portion of the biologic sample into each of the first plurality of pathways. A second plurality of pathways is provided, each for determining a dosage level of a particular one of the plurality of treatment agents with respect to the predetermined biologic material. A plurality of second micro-pumps are provided for pumping a second portion of the biologic sample into a selected one of the second plurality of pathways responsive to the determination of treatment efficacy of the treatment agent providing a best treatment of the predetermined biologic material.

Provided is a device including a well provided in a number of at least one, wherein a nucleic acid having at least one of a full-length nucleotide sequence and a partial nucleotide sequence of rRNA or rDNA is contained in a defined copy number in at least one well of the well, and wherein the defined copy number of the nucleic acid having at least one of a full-length nucleotide sequence and a partial nucleotide sequence of rRNA or rDNA is 1,000 or less.

At least one embodiment relates to a focusing arrangement for focusing particles or cells in a flow. The arrangement includes at least one channel for guiding the flow. The channel includes (i) at least one particle confinement structure having particle flow boundaries and (ii) at least one acoustic confinement structure having acoustic field boundaries adapted for confining acoustic fields. The acoustic field boundaries may be different from the particle flow boundaries, and the at least one acoustic confinement structure may be arranged with regard to the channel to at least partially confine acoustic fields in the channel.