The invention relates to an integrated tubular reaction device, which comprises a reaction vessel, a reaction vessel including at least two tubular chambers, a channel connecting at least two tubular chambers and an opening; a cover body, which can be worked with the opening, and a cover body including a through hole; a seal, which includes a sealing plug which can be worked with the through hole. The integrated tubular reaction device solves the problem of contamination of reaction products in the process of multiple or multi-step biological enzyme reaction, and can realize multiple or multi-step biological enzyme reactions in the same device.

The present invention discloses an apparatus for performing PCR by thermal convection. The device includes a first bracket, a second bracket, a temperature sensing device, a power supplying device, a processor and an accommodation space. The device uses transparent conductive film to replace conventional thermostat metal stock to perform a heat process required in the PCR or RT-PCR process. The device further uses a reagent container whose bottom portion contacts the transparent conductive film and being heated by the transparent conductive film to establish a thermal circulation in the reagent container. The device can qualify or quantify the product of PCR or RT-PCR process by further incorporating specific probes, fluorescence material, light source, light receiver and light regulator.

A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

A method for amplifying nucleic acids by a polymerase chain reaction in a reaction volume heated using electrical energy. In at least one of the passages of the amplification cycle of the polymerase chain reaction, the ratio of the electrical energy used in the denaturation step to heat the reaction volume to the size of the reaction volume is less than 20 Joule per milliliter. Further shown is a method of amplifying nucleic acids in a reaction volume by using a device that includes a reaction vessel and a heating means with at least one heating element in contact with the reaction volume where at least one heating element is conjugated to oligonucleotides. Also shown is a device for the amplification of nucleic acids in a reaction volume including a reaction vessel for receiving the reaction volume and a heating means consisting of at least one heating element contacting the reaction volume.

A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

An automated method for performing an assay of the present disclosure can be performed in a microfluidic device that is a lateral flow device having numerous features to ensure correct operation of the device under gravity, such as vent pockets for enabling the flow of sample fluid from one chamber to the next when the vent pocket is unsealed. Each chamber can have a reagent recess proximal to an inlet end of the chamber. A reagent pellet formed in a reagent recess can be effectively mixed with a sample as the sample flows into the chamber. A flexible circuit with patterned metallic electrical components disposed on a heat stable material can be in direct contact with fluid in the chambers and has resistive heating elements aligned with, for example, a chamber for performing an amplification reaction. A lateral flow detection chamber can include a capillary pool proximal to a sample receiving end of a lateral flow strip, providing effective mixing and dispersion of a sample with detection particles, as well as enhancing, uniformity of particle migration on the detection strip. The microfluidic device can be configured to be hermetically sealed, thereby preventing contamination of a testing environment.

A thermal convection generating chip (1) includes a rotatory body (2), a thermal convection pathway (11) provided in the rotatory body (2), and a supply path (12A to 12C) that supplies a liquid to the thermal convection pathway (11). The supply path (12A to 12C) includes a liquid receiving section (121) that receives the liquid and a suction passage (122) that provides communication between the liquid receiving section (121) and the thermal convection pathway (11). The suction passage (122) has a first region (122a) extending between a midsection of the suction passage (122) and the thermal convection pathway (11), and a second region (122b) extending between the midsection and the liquid receiving section (121). The liquid in the first region (122a) is separated from the liquid in the second region (122b) through rotation of the rotatory body (2) to be supplied to the thermal convection pathway (11).

Disclosed are a reaction tube for nucleic acid amplification capable of controlling a liquid circulation path, a reaction apparatus for nucleic acid amplification comprising the reaction tube, and a method for amplifying nucleic acid comprising a step of using the reaction tube. Also disclosed are a kit comprising the reaction tube, and use of the reaction tube in preparation of a kit.

A system and method for isolating and analyzing single cells, comprising: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

The present invention refers to a device for the analysis of a particle comprising an analysis chamber adapted to contain a positioning fluid. A parameter of the particle suspended in the positioning fluid is detected by means of a detection and control unit. A positioning unit, during a particle analysis operation, is activated and deactivated on the basis of the detected parameter of the particle. The detection and control unit can activate the at least one positioning unit so as to generate a temporary positioning flow in the positioning fluid, such that said temporary positioning flow acts directly on the particle and drives the position of the particle so as to move it into a predefined position in the analysis chamber. The detection and control unit can also deactivate the at least one positioning unit when the particle to be analyzed is in the predefined position, such that the positioning fluid is at rest.