A microfluidic assembly may include a microfluidic chip operably coupled to a device source pressure port and a device relief pressure port, first and second input reservoirs, an output reservoir, and a reservoir interface. The microfluidic chip may include a microfluidic circuit configured to support a fluid flow that includes a gas flow and a liquid flow within the microfluidic circuit. The reservoir interface may be configured to operably couple the first and second input reservoirs to the microfluidic circuit. The device source pressure port may be configured to receive a source pressure to generate the fluid flow through the microfluidic circuit and cause a mixing of liquids to form an output liquid for delivery to the output reservoir via the fluid flow. The first liquid, the second liquid, and the output liquid need not contact the device source pressure port or the device relief pressure port during the mixing.

Diagnostic systems for sensing biological molecules are disclosed. The diagnostic systems include a fluid control delivery and control system that can be coupled to a diagnostic cartridge that includes a microfluidic device and integrated sensing electronics. The diagnostic systems can be used to sequence biological molecules.

Provided are handling systems and methods for sample processing. The systems can include a sample container and a probe. A system can be convertible between a loading state in which a user loads sample into the sample container, a sampling state in which relative motion between the sample container and the probe places the probe and sample container into fluid communication with one another so that the probe can aspirate sample from the sample container, and a washing state in which rinse fluid is expressed through the probe so as to remove unwanted material from within the probe, with the rinse fluid being collected by a wash manifold.

An in-vitro diagnostic analyzer, a reagent card (10), and an installation structure (200) are disclosed. The installation structure (200) includes an installation body (210). The installation body (210) includes an installation hole (212) configured to sleeve a sample tube (70), a hollow needle (220), a sealing portion (240), and an air inlet channel (230). One end of the hollow needle (220) is capable of being inserted into the sample tube (70). The sealing portion (240) is in sealing fit with an outer wall of the sample tube (70). The air inlet channel (230) includes an air outlet hole (234) and an air inlet hole (232). The air outlet hole (234) is configured for communication with the sample tube (70) provided on the installation hole (212). The reagent card (10) is integrated with the installation structure (200), and the in-vitro diagnostic analyzer is integrated with the reagent card (10).

Disclosed in one aspect is a method for performing a complete blood count (CBC) on a sample of whole blood by metering a predetermined amount of the whole blood and mixing it with a predetermined amount of diluent and stain and transferring a portion thereof to an imaging chamber of fixed dimensions and utilizing an automated microscope with digital camera and cell counting and recognition software to count every white blood cell and red blood corpuscle and platelet in the sample diluent/stain mixture to determine the number of red cells, white cells, and platelets per unit volume, and analyzing the white cells with cell recognition software to classify them.

A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.

Methods, systems and devices that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer.

The invention provides a device and method for the real-time detection of aeropathogens. The device includes an aerosampler having an air inlet and at least one collector tube, a microfluidic system which includes a container, piping, a micro pump for flowing a liquid and a viral detection chamber. The viral detection chamber has an electrode which may be equipped with functionalized bio sensors, a counter electrode, an electronic detection system connectable to the electrodes of the viral detection chamber, and an embedded electronic processing system for processing data from the electronic detection system.

Some embodiments of a micro-fluidic device include at least one inlet hole located on an inlet side of the microfluidic device, the inlet hole consisting of a plurality of holes with diameters smaller in size than a diameter of the at least one inlet hole, at least one outlet hole located on an outlet side of the microfluidic device opposite the inlet side; and a micro-channel, where the plurality of holes are connected to the micro-channel.