A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

The invention relates to a connection method in addition to a functional part which can be used therefor and to a flame-retardant total system produced in accordance therewith. The invention relates to a method for connecting a single- or multilayer functional part (10) to a third component (20), wherein the functional part (10) has functional elements (14, 16) projecting on at least one side. Said method is characterized in that the functional part (10) is formed at least partially from a material which is at least partially transparent to laser light, and in that a blocking layer (18) is disposed so as to be at least partially opaque to laser light in such a way that after passage of the laser light through the functional part (10) the laser light inside the blocking layer (18) generates heat by absorption which is suitable for melting the functional part (10) and/or the third component (20) in order to connect them to one another in this way.

A method can include providing two or more parts. The two or more parts can include a first part and a second part. The method can include disposing a source material between the first part and the second part. The method can include joining and densifying the first part and the second part by reacting a liquid with the source material.

A process for fixing an article to a substrate with a mounting set including two receptacles storing an aerobic adhesive and a hydrophilic substance, respectively. The aerobic adhesive and the hydrophilic substance are removed from the two receptacles and then mixed together to prepare the fastening. A layer of the fastening composition is applied to the article. The article is fixed to the substrate by pressing the layer of the fastening composition against the substrate. The aerobic adhesive in the layer hardens because of the moisture contained in the hydrophilic substance.

A technique for minimising blooming or frosting that may occur when bonding sub-components of an article together to form a composite component with an adhesive such as cyanoacrylate. Opposed bonding regions on surfaces of first and second sub-components are bonded by the adhesive and at least one of the opposed bonding regions is provided with a depression in its surface which is capable of receiving and containing an amount of uncured/curing adhesive. The depression is preferably shaped and dimensioned so as to contain the uncured adhesive while minimising the adhesive's surface area exposed to the atmosphere. The depression may be shaped as an elongate groove along at least a part of a bonding region, such as along at least a part of the periphery of a bonding region.

Barrier systems for building structures are provided. A system comprises a building structure, a first membrane, a second membrane, and a cured barrier membrane spray film. The first membrane and the second membrane are located on the building structure, such that the second membrane partially overlaps the first membrane in an overlapping portion. The cured barrier membrane spray film is located between the first membrane and the second membrane in the overlapping portion. Related systems, related aerosilizable compositions, and related methods are provided.