Provided herein are methods for producing a mung bean protein isolate having high functionality for a broad range of food applications. In some embodiments, the methods for producing the isolate comprise one or more steps selected from: (a) extracting one or more mung bean proteins from a mung bean protein source in an aqueous solution, for example, at a pH between about 6.5-10.0; (b) purifying protein from the extract using at least one of two methods: (i) precipitating protein from the extract at a pH near the isoelectric point of a globulin-rich fraction, for example a pH between about 5.0-6.0; and/or (ii) fractionating and concentrating protein from the extract using filtration such as microfiltration, ultrafiltration or ion-exchange chromatography; and (c) recovering purified protein isolate.

This invention relates to a food product comprising an additive formulation, the additive formulation comprising a proteinaceous microgel and a biopolymeric hydrogel. The invention also relates to methods for preparing such food products. The invention also relates to a use of an additive formulation as a fat replacement and/or an astringency reducer and/or a mouthfeel enhancer, the additive formulation comprising a proteinaceous microgel and a biopolymeric hydrogel.

The present disclosure provides a human milk fortifier product composition with, a protein component in the milk fortifier in an amount ranging from 6 wt/wt % to 35 wt/wt % on solids basis; a fat component in the in the milk fortifier in an amount ranging from 0.1 wt/wt % to 36 wt/wt % on solids basis; a carbohydrate in the milk fortifier selected from the group of Lactose and Oligosaccharides present in the in an amount from 25 wt/wt % to 75 wt/wt % on solids basis; small molecules consisting of Oligosaccharides in the human milk fortified by the said fortifier is at least 25% more than the human milk alone. The majority of small molecules consisting of Vitamin A, Vitamin D, Vitamin E, LCPUFA, Epidermal growth factor and many others are retained in the fortifier product.

Featured are a foodstuff containing an inter-alpha inhibitor protein (IIp) (e.g., II, PI, a heavy chain (e.g., H1, H2, H3, H4, and/or H5), a light chain (e.g., bikunin), or a combination thereof), methods of preparing a foodstuff containing an IIp, and methods of treating and/or reducing the likelihood of developing disease or condition (e.g., a disease or condition characterized by inflammation and/or low levels of an IIp) in a subject in need thereof by administering a composition or a foodstuff containing an IIp. Also featured are methods of purifying an IIp (e.g., II, PI, a heavy chain (e.g., H1, H2, H3, H4, and/or H5), a light chain (e.g., bikunin), or a combination thereof) from milk.