The present invention relates to a method of producing a coffee extract wherein coffee beans are contacted with cellulase and xylanase.

Disclosed are processes for making cold brew beverage compositions including concentrates and apparatus for preparing cold brew beverage compositions. The disclosed processes and apparatus allow for efficient, rapid, industrial scale production of cold brew beverage compositions having improved total solid content, improved flavor stability, longer shelf-life, and adaptability to use in small volume containers.

The invention relates to compositions for preparing a beverage, the composition comprises a) a coffee liquor or dried coffee extract ranging between 1-40% w/w; b) a creamer ranging between 1-20% w/w; c) a viscosity modifier ranging between 1-15 w/w %; d) an emulsifier ranging between 0.1 to 1%; and e) a -mannanase ranging between 0.1 to 1% w/w.

The present invention relates to a method for producing a coffee extract which comprises use of an enzyme having mannanase activity. The invention also relates to polypeptides having endo-beta-1,4-mannanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to a method for producing a coffee extract which comprises use of an enzyme having mannanase activity. The invention also relates to polypeptides having endo-beta-1,4-mannanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.

The purpose of the present invention is to provide a technique for producing a coffee extract, the technique being capable of further reducing the turbidity of the coffee extract. Provided are: a method which is for producing a coffee extract with reduced turbidity and includes a step for bringing a coffee extract into contact with glucoamylase of which the glucoamylase activity per 1 g of coffee beans is 32 U or less; and a method which is for producing a coffee extract with reduced turbidity and includes a step for bringing a coffee extract into contact with glucoamylase and galactomannanase, wherein the glucoamylase is used such that the glucoamylase activity is 0.24 U or more per 1 U of the galactomannanase activity.

The present invention relates to polypeptides having beta-1,3-galactanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The invention provides a method of processing green coffee beans that improves the aromas and flavors of green coffee beans through simple means, allowing richly fragrant green coffee beans that have unique aromas to be obtained with ease. A method of processing green coffee beans includes a fermentation process by bringing green coffee beans, which are unground seeds from coffee berries, microorganisms, and nutritive substances that are metabolized by the microorganisms into contact with one another to cause fermentation, followed by a separation process of separating out only said green coffee beans that have passed through the fermentation process.

Disclosed herein is a process for production of an instant coffee product in a high yield, comprising the steps of extracting roast and ground coffee beans with water having a temperature of 80 C. or less, to produce a first extract and spent coffee grounds, adding water to the spent coffee grounds to produce an aqueous suspension, hydrolysing the aqueous suspension using a hydrolysing enzyme to produce a second extract and spent remains, adding the first extract to the second extract, optionally after concentration and/or drying of the second extract, to obtain a combined extract, and drying the combined extract to obtain an instant coffee product. The high yield is obtained due to the reduction of enzyme inhibiting substances.