The present disclosure relates to food compositions, particularly pet food compositions, having a high content of plant protein. The plant protein is prepared by processing Brewer's Spent Grain (BSG), having a moisture content of about 15% to about 35% by weight, so as to reduce the particle size of the BSG and separating the processed BSG so as to provide a low particle size, high protein fraction.

A process prepares a beverage or beverage component. The process includes an enzymatic treatment of brewer's spent grain including addition of one or a combination of enzymes with alpha-amylase, gluco-amylase, cellulase, xylanase, protease and/or beta-glucanase activity and fermentation by a strain of lactic acid bacteria. The combination of enzymes and enzymatic treatment conditions is such that the lactic acid bacteria produce 4.5 g/L lactic acid and metabolise sugar such that the resulting fermented broth contains less than 2.5% w/w and preferably less than 0.5% w/w residual sugar or the lactic acid bacteria produce 4.5 g/L lactic acid and metabolise sugar such that the resulting fermented broth contains at least 2.5% w/w residual sugar.

Systems and methods for system for upcycling solid food wastes and by-products into food-grade nutritional products and the high-protein nutritional product manufactured by the systems and methods are described herein. A system for upcycling solid food wastes and by-products into food-grade nutritional products may include an extruder. The extruder may be configured to receive a raw plant-based material and continuously subject the raw plant-based material to steam flashing at predetermined temperature and predetermined pressure to sterilize the raw plant-based material, destroy antinutrients present in the raw plant-based material, cause a break up of larger cellular and subcellular units of the raw plant-based material into smaller cellular and subcellular units of the raw plant-based material to transform the raw plant-based material into a food ingredient, and reduce water content in the food ingredient.

This disclosure describes methods to separate solids from liquids in a production facility. A process separates components in the process stream by applying non-condensable media to create density differences and then using a mechanical device to separate the solids from the liquids based on the density difference. The process produces the liquids and solids, which may be further processed to create valuable animal feed products.

A multi stage process for the separation of bio-components from a waste stream containing Dried Distillers Grains with Solubles is disclosed. Targeted polymers are added to the source and separated streams prior to passing the streams through separation equipment including a rotary screen, a press, and a dissolved air floatation in which the waste stream is separated into a stream containing predominantly protein, a stream containing predominantly oil, a stream containing predominantly water and a stream that contains predominantly fibers.

A protein product and an extraction method for making the protein product. Raw materials of the product optionally contains macromolecular carbohydrates and/or fat, and the method does not use organic solvents. The protein product contains prolamin and carbohydrates, wherein the prolamin accounts for 70 wt % or above of the protein (dry-basis), the -prolamin accounts for 75 wt % or above of the prolamin, the -prolamin accounts for 20 wt % or below of the prolamin, and the -prolamin accounts for 6 wt % or below of the prolamin.

Provided is a method of protein recovery from a plant by-product. The method comprises isoelectric solubilisation of the plant by-product with an alkali solution to provide a first solubilised protein fraction, separating the first solubilised protein fraction from unsolubilised plant matter; and flocculating the first solubilised protein fraction with an amount of a flocculating agent to provide a protein product.

The invention pertains to a method for processing aleurone-containing plant-based material characterized in that the method comprises the steps of: i) optionally washing the aleurone-containing plant-based material, whereby protein is separated from the aleurone-containing plant-based material to obtain a first protein-containing liquid (22) and the aleurone-containing plant-based material: ii) optionally removing water from the aleurone-containing plant-based material to obtain an aleurone-containing plant-based material suspension (2) containing 1 to 30% by weight of the aleurone-containing plant-based material, based on the total weight of the suspension, and obtaining a second protein-containing liquid (23); and ill) treating the suspension of the aleurone-containing plant-based material (2) containing 1 to 30% by weight of the aleurone-containing plant-based material with a high shear mill (3) to at least partially release protein encapsulated in the aleurone cells, and to obtain a ground aleurone-containing plant-based fiber (4).

The present invention provides the method and means for an efficient isolation of membrane-bound proteins from biological samples, e.g. in samples from raw or processed plant material, preferably defatted plant seed meal such as canola meal. The biological sample can be highly processed, e.g. by applying high temperature, pressure, or a chemical treatment and can be derived from seed matrices as well as other typical plant tissues for example seed, grain, leaf, root, or pollen. The invention comprises the provision of a novel extraction buffer (MEB) and its application in the method of the invention, wherein the buffer has a strong alkaline pH of 10 to 12.5 and comprises a soluble concentration of detergent at a level of 0.5% to 5%.

The present disclosure relates to a method for producing a high moisture texturised plant protein. The method comprises the steps of a) grinding of brewer's spent grain, BSG, b) providing ingredients for obtaining a mixture, the ingredients comprising 15% to 60% by weight of the ground BSG and at least a plant protein, c) processing the mixture in an extruder, wherein a homogenous fibrous structure is generated in the extruder to obtain a texturised plant protein, d) first cooling of the texturised plant protein, and e) cutting the texturised plant protein.