A method for preparing IPP- and VPP-rich hydrolysate from wheat gluten protein by enzymatic hydrolysis is disclosed, comprising the following steps: (1) dissolving wheat gluten protein in an alkaline solution, and performing ultrasonic treatment; (2) adding the alkaline protease to the wheat gluten protein solution to hydrolyze; (3) adjusting the pH value of the alkaline hydrolysate to neutral or weakly acidic, and adding protease to hydrolyze, and inactivating the enzyme at a high temperature; (4) centrifuging the hydrolysate to obtain the supernatant, and ultrafiltrating with an ultrafiltration membrane; (5) decolorizing and debitterizing the permeate using a macroporous resin; (6) after the debittered and decolorized liquid is adsorbed by an ion exchange resin, eluting the ion exchange resin and drying, to obtain a powder of IPP- and VPP-rich hydrolysate. The preparation method has a high protein recovery rate, with rich IPP and VPP in the hydrolysate.

A method for preparing IPP- and VPP-rich hydrolysate from wheat gluten protein by enzymatic hydrolysis is disclosed, comprising the following steps: (1) dissolving wheat gluten protein in an alkaline solution, and performing ultrasonic treatment; (2) adding the alkaline protease to the wheat gluten protein solution to hydrolyze; (3) adjusting the pH value of the alkaline hydrolysate to neutral or weakly acidic, and adding protease to hydrolyze, and inactivating the enzyme at a high temperature; (4) centrifuging the hydrolysate to obtain the supernatant, and ultrafiltrating with an ultrafiltration membrane; (5) decolorizing and debitterizing the permeate using a macroporous resin; (6) after the debittered and decolorized liquid is adsorbed by an ion exchange resin, eluting the ion exchange resin and drying, to obtain a powder of IPP- and VPP-rich hydrolysate. The preparation method has a high protein recovery rate, with rich IPP and VPP in the hydrolysate.

The present invention describes a bio-based process to produce high quality protein concentrate (HQPC) by converting plant derived celluloses into bioavailable protein via aerobic incubation, including the use of such HQPC so produced as a nutrient, including use as a fish meal replacement in aquaculture diets.

Provided are improved methods for treating wheat flour with lipase. Further provided are methods for separating wheat flour to provide a gluten fraction, a starch fraction and a fibre fraction where the wheat flour is treated with a selected lipase. Polypeptides having lipase activity, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides are also provided.

The present invention relates to use of a laccase for controlled enzymatic browning of a meat analogue product comprising a red pigment which changes colour when oxidized. Preferably, the laccase is embedded in a fat having a melting temperature of at least 25° C. such that the oxidation of the red pigment occurs only at elevated temperature, e.g. during cooking of the meat analogue product.

A method for improving the proteinaceous fibre structure of a textured vegetable protein product, methods of controlling the mouthfeel of a textured protein, and textured vegetable protein products. In the method for improving the proteinaceous fibre structure of a textured vegetable protein product, an extrudate is prepared with an extruder configured to carry out low-moisture protein texturization extrusion, the extrudate including a proteinaceous fibre structure having expansion-related cavities, such as air bubbles, between the proteinaceous fibres; after the extrusion, the extrudate is compressed or compacted in a manner leaving the proteinaceous fibres of the extrudate substantially intact. The compressing or compacting is sustained over a period that causes an irreversible reduction in the size of the expansion-related cavities between the proteinaceous fibres, and preferably also an increase in the bonding between the proteinaceous fibres.

A method for improving the proteinaceous fibre structure of a textured vegetable protein product, methods of controlling the mouthfeel of a textured protein, and textured vegetable protein products. In the method for improving the proteinaceous fibre structure of a textured vegetable protein product, an extrudate is prepared with an extruder configured to carry out low-moisture protein texturization extrusion, the extrudate including a proteinaceous fibre structure having expansion-related cavities, such as air bubbles, between the proteinaceous fibres; after the extrusion, the extrudate is compressed or compacted in a manner leaving the proteinaceous fibres of the extrudate substantially intact. The compressing or compacting is sustained over a period that causes an irreversible reduction in the size of the expansion-related cavities between the proteinaceous fibres, and preferably also an increase in the bonding between the proteinaceous fibres.

A method of producing a hybrid foodstuff is provided. The method comprises combining a plant-originated substance with an amount of cultured animal cells so as to enhance a meat organoleptic and/or meat nutritional property in the hybrid foodstuff, wherein the animal cells do not form a tissue, and wherein the amount is below 30% (w/w) of the hybrid foodstuff.

A method of producing a hybrid foodstuff is provided. The method comprises combining a plant-originated substance with an amount of cultured animal cells so as to enhance a meat organoleptic and/or meat nutritional property in the hybrid foodstuff, wherein the animal cells do not form a tissue, and wherein the amount is below 30% (w/w) of the hybrid foodstuff.

A method of producing a hybrid foodstuff is provided. The method comprises combining a plant-originated substance with an amount of cultured animal cells so as to enhance a meat organoleptic and/or meat nutritional property in the hybrid foodstuff, wherein the animal cells do not form a tissue, and wherein the amount is below 30% (w/w) of the hybrid foodstuff.