Disclosed are methods of administering one or more Bacillus subtilis strains to swine. The Bacillus subtilis strains that are administered include 747 (NRRL B-67257), 1104 (NRRL B-67258), 1541 (NRRL B-67260), 1781 (NRRL B-67259), 2018 (NRRL B-67261), and BS1999 (NRRL B-67318). The Bacillus strains improve bacterial homeostasis in the gastrointestinal tract by inhibiting bacterial pathogens such as E. coli, Clostridium, Salmonella, and Streptococcus. Administering the Bacillus strains also improves performance such as weight gain and feed conversion. Useful combinations of Bacillus strains and methods of using one or more Bacillus strains are also provided.

A process for converting invertebrates into a feedstock or feedstocks using microbial fermentation, and products thereof.

A process for converting invertebrates into a feedstock or feedstocks using microbial fermentation, and products thereof.

Provided is a method of preparing a granular feed additive. When the method of preparing a granular feed additive according to one exemplary embodiment is used, it is possible to prepare a granular feed additive including a high content of basic amino acids while preventing hygroscopicity and agglomeration caused by the basic amino acids. In addition, since the method of preparing a granular feed additive according to one exemplary embodiment may omit a process of using hydrochloric acid, which is generally used to neutralize basic amino acids, it is possible to simplify the process and to solve process problems caused by the use of hydrochloric acid.

A traditional Chinese medicine polysaccharide extract freeze-drying protective agent, a direct-to-vat starter and preparation methods thereof are disclosed, which belong to the technical field of feed starter. The compound traditional Chinese medicine polysaccharide extract freeze-drying protective agent includes Poria cocos polysaccharide extract, Atractylodes macrocephala polysaccharide extract, Radix codonopsis polysaccharide extract, L-tyrosine, α-cyclodextrin and polyethyleneimine. Its application in the freeze-drying protection of direct-to-vat starter of aquatic fermentation feed can reduce the damage to the cells caused by freeze-drying, improve the survival rate of the cells in the starter after freeze-drying, effectively prolong the storage period of the starter, and ensure that the fermentation activity remains at a high level during storage.

A traditional Chinese medicine polysaccharide extract freeze-drying protective agent, a direct-to-vat starter and preparation methods thereof are disclosed, which belong to the technical field of feed starter. The compound traditional Chinese medicine polysaccharide extract freeze-drying protective agent includes Poria cocos polysaccharide extract, Atractylodes macrocephala polysaccharide extract, Radix codonopsis polysaccharide extract, L-tyrosine, α-cyclodextrin and polyethyleneimine. Its application in the freeze-drying protection of direct-to-vat starter of aquatic fermentation feed can reduce the damage to the cells caused by freeze-drying, improve the survival rate of the cells in the starter after freeze-drying, effectively prolong the storage period of the starter, and ensure that the fermentation activity remains at a high level during storage.

Disclosed are methods of dewatering solid byproduct. In some embodiments, the solid byproduct contains particles and is produced from a fermentation process for making an oxygenated compound such as ethanol. The method comprises a chemical sequence for conditioning (pre-treating) the solid byproduct to be dewatered. The solid byproduct (in water) is treated with alkaline material to increase its pH to about 7-8.5. Coagulant is added to the alkaline-treated solid byproduct to reduce charge on the solid byproduct. An agglomerating polymer is then added to increase the average size of the solid byproduct particles to a desired size (e.g., at least about 1 mm). Dewatering can further use known technologies such as screw press, belt press, filter press, centrifuge, and/or a dryer to separate the conditioned or pre-treated byproduct from water. Also disclosed are methods of producing oxygenated product, as well as methods of producing animal feed and/or fertilizer, respectively.

The present invention provides a method for producing a biomass comprising at least 65% protein from hydrogen-oxidising microorganisms using one or more input streams comprising one or more gaseous substrates and a nutrient composition which are controlled and wherein the biomass is produced at a rate of more than 10 g/l/d. The produced and isolated biomass is useful as feed or to provide nutrition to one or more organisms.

The present invention provides a method for producing a biomass comprising at least 65% protein from hydrogen-oxidising microorganisms using one or more input streams comprising one or more gaseous substrates and a nutrient composition which are controlled and wherein the biomass is produced at a rate of more than 10 g/l/d. The produced and isolated biomass is useful as feed or to provide nutrition to one or more organisms.

Reactor assemblies and methods for hydrolytic biomass conversion are disclosed herein. The reactor assemblies employ solely hydrostatic pressure, applied by the liquid biomass slurry, as means of providing pressure necessary to maintain high temperature conditions suitable for hydrolytic biomass conversion resulting in the formation of soluble pentose compounds. In embodiments, the methods employed in conjunction with the reactor assemblies yield soluble C.sub.5 carbohydrates in the forms of oligosaccharides and monosaccharides from one or more biomass slurries.