The application relates to a preparation method of liquid bio-fermented feed, liquid bio-fermented feed and bio-fermented emulsion liquid feed, belonging to the technical field of feed preparation. The preparation method of the present application may overcome physiological problems of weaned piglets such as insufficient gastric acid secretion, low content and activity of digestive enzymes, incomplete development of immune system, disorder of intestinal microecosystem, and the like. The prepared bio-fermented liquid feed has good palatability and high nutrition, easy digestion, low acidity, and is a liquid feed similar to breast milk, which can both effectively alleviate the weaning stress syndrome of piglets, and effect a seamless transition from breast milk to conventional solid feed.

Methods, compositions, and systems for steeping, propagation and fermentation, particularly large-scale operations for production of starch and ethanol and fermentation product streams are provided. Addition of mycotoxin mitigating enzymes or microorganisms expressing mycotoxin mitigating enzymes to steeping, propagation, and/or fermentation tanks, and/or to post-fermentation product streams, mitigates mycotoxin levels in fermentation co-products obtained from mycotoxin contaminated feedstocks.

Methods, compositions, and systems for steeping, propagation and fermentation, particularly large-scale operations for production of starch and ethanol and fermentation product streams are provided. Addition of mycotoxin mitigating enzymes or microorganisms expressing mycotoxin mitigating enzymes to steeping, propagation, and/or fermentation tanks, and/or to post-fermentation product streams, mitigates mycotoxin levels in fermentation co-products obtained from mycotoxin contaminated feedstocks.

The present disclosure relates to selective analytical methods for the detection and/or quantification of an oligosaccharide preparation in a nutritional composition such as an animal feed. Also disclosed are methods of manufacturing a nutritional composition comprising an oligosaccharide preparation, the presence or concentration of which can be selectively detected or determined.

The present invention relates to a method for increasing the stability of an α/β-hydrolase. In addition, the present invention relates to an α/β-hydrolase obtainable by the method of the present invention. Also provided are α/β-hydrolases having a decreased grand average of hydropathy (GRAVY) value and/or comprising specific mutations. In addition, the present invention concerns a use of an α/β-hydrolase of the present invention for degrading zearalenone (ZEN).

Improvement of the intestinal health of a monogastric animal by increasing the levels of cecal butyrate levels in situ in said animal by administering a GH30 glucuronoxylan hydrolase-enriched maize-based animal feed is described. Improving the feed conversion ratio of said animal feed is furthermore observed.

Improvement of the intestinal health of a monogastric animal by increasing the levels of cecal butyrate levels in situ in said animal by administering a GH30 glucuronoxylan hydrolase-enriched maize-based animal feed is described. Improving the feed conversion ratio of said animal feed is furthermore observed.

A dog treat made from a dairy based product, preferably milk having a fat content of approximately one percent, which is heated with other ingredients and pressed and hardened into a desired configuration to form a hardened cheese-like dog treat.

A dog treat made from a dairy based product, preferably milk having a fat content of approximately one percent, which is heated with other ingredients and pressed and hardened into a desired configuration to form a hardened cheese-like dog treat.

A method for the production of a hydrolysate comprising: (a) admixing at least one protein or a portion thereof with: (A) at least one endoprotease; and (B) (a′) at least one proline tolerant tripeptidyl peptidase or fermentate comprising a proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at P1; and synthetic amino acids at P1; or (b′) at least one proline tolerant tripeptidyl peptidase having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having: Proline at PV; and synthetic amino acids at PV; and (b) recovering the hydrolysate. The invention also relates to methods for producing a hydrolysate comprising the use of an endoprotease an exo-tripeptidyl peptidase of the S53 family and an aminopeptidase, to uses of a proline tolerant tripeptidyl peptidase, compositions, food and/or feed additive compositions comprising the same, as well as hydrolysates and uses of proline tolerant tripeptidyl peptidases.