The present disclosure is directed to an animal food product for promoting dental efficacy including a hydrolyzed protein including one or more amino groups, and a high amylopectin content starch including one or more reducing sugars; wherein the one or more amino groups in the hydrolyzed protein are covalently bonded to the one or more reducing sugars in the high amylopectin content starch. Methods of preparing the animal food product and using the animal food product to promote dental efficacy, such as teeth-cleaning efficacy in an animal are also provided.

A new alga having an improved ability to produce a pigment is disclosed. When the alga is used, a carotenoid-based pigment, specifically, a xanthophyll can be produced by consuming less energy, so that it is possible to efficiently produce the pigment at the industrial level. The pigment can be applied as a raw material for a food, a health functional food and a medicine, which include the pigment. Since a DNA fragment is not likely to be inserted into a target base sequence or a base sequence other than the target, it is expected that the procedure of constructing the mutant is not regulated as a GMO.

A new alga having an improved ability to produce a pigment is disclosed. When the alga is used, a carotenoid-based pigment, specifically, a xanthophyll can be produced by consuming less energy, so that it is possible to efficiently produce the pigment at the industrial level. The pigment can be applied as a raw material for a food, a health functional food and a medicine, which include the pigment. Since a DNA fragment is not likely to be inserted into a target base sequence or a base sequence other than the target, it is expected that the procedure of constructing the mutant is not regulated as a GMO.

A new alga having an improved ability to produce a pigment is disclosed. When the new alga is used, a carotenoid-based pigment, specifically, a xanthophyll can be produced by consuming less energy, so that it is possible to efficiently produce the pigment at the industrial level. The pigment can be applied as a raw material for a food, a health functional food and a medicine, which include the pigment. Since a DNA fragment is not likely to be inserted into a target base sequence or a base sequence other than the target, it is expected that the procedure of constructing the mutant is not regulated as a GMO, so that it is expected that the procedure of constructing the mutant can create a big economic effect in terms of an industry which produces lutein and zeaxanthin by using microalgae.

A new alga having an improved ability to produce a pigment is disclosed. When the new alga is used, a carotenoid-based pigment, specifically, a xanthophyll can be produced by consuming less energy, so that it is possible to efficiently produce the pigment at the industrial level. The pigment can be applied as a raw material for a food, a health functional food and a medicine, which include the pigment. Since a DNA fragment is not likely to be inserted into a target base sequence or a base sequence other than the target, it is expected that the procedure of constructing the mutant is not regulated as a GMO, so that it is expected that the procedure of constructing the mutant can create a big economic effect in terms of an industry which produces lutein and zeaxanthin by using microalgae.

A method for producing a carotenoid enriched fatty acid composition includes: reacting an oil including free fatty acids and carotenoids with a basic solution; withdrawing, separately from the oil, an extraction solution including at least a portion of the free fatty acids, at least a portion of the carotenoids, and the basic solution; acidifying the extraction solution to produce an aqueous phase and a fatty acid phase, the fatty acid phase including the free fatty acids and the carotenoids of the extraction solution; and separating the fatty acid phase from the aqueous phase.

A method for producing a carotenoid enriched fatty acid composition includes: reacting an oil including free fatty acids and carotenoids with a basic solution; withdrawing, separately from the oil, an extraction solution including at least a portion of the free fatty acids, at least a portion of the carotenoids, and the basic solution; acidifying the extraction solution to produce an aqueous phase and a fatty acid phase, the fatty acid phase including the free fatty acids and the carotenoids of the extraction solution; and separating the fatty acid phase from the aqueous phase.

The present invention provides improved processes for extracting and preparing lipids from biological sources for use in pharmaceuticals, nutraceuticals and functional foods.

The present invention provides improved processes for extracting and preparing lipids from biological sources for use in pharmaceuticals, nutraceuticals and functional foods.

Disclosed are methods of producing carotenoid compounds in a methylotrophic bacterial host cell. Such a host cell may be an unmodified Methylobacterium, spontaneous mutant, or transformed cell, any of which exhibit favorable properties, such as overproduction of carotenoid compounds, increased carbon flux, improved growth, or the production of additional nutrients, such as protein, vitamins, antioxidants, or fatty acids. Also disclosed are feed compositions for use in aquaculture, or as animal feed, or as human nutritional supplements containing processed or unprocessed biomass from such cells, as are methods of preparation of the feed compositions.