The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed.

A consumable having replenishment properties to be consumed by animals to maintain and/or restore proper hydration and electrolyte levels includes a base portion and an electrolyte portion. The base portion is processed by at least one of pureeing, juicing, liquefying and macerating. The base portion can include one or more of an item of produce. The base portion can be all-natural. The electrolyte portion can include one or more of a sugar, potassium and sodium. The electrolyte portion is combined with the base portion to generate the consumable. The consumable can also include a natural supplement such as vitamins, minerals and/or proteins. The consumable has a melting point and may be frozen prior to consumption or may be consumed in its liquid-like or slurry-like form.

The invention is directed to a potassium containing preparation or potassium bolus for the prevention or treatment of potassium deficit and optionally other deficit of minerals in ruminants, a process for the production thereof as well as the use thereof. The present invention makes it possible to provide a potassium containing preparation or potassium bolus with a high content of up to 70 wt.-% of potassium salt, based on the total weight of the potassium containing preparation, by adding only a relatively small amount of calcium chloride (15 to 40 wt.-%) and water (10 to 22 wt.-%) to the potassium salt.

The invention is directed to a potassium containing preparation or potassium bolus for the prevention or treatment of potassium deficit and optionally other deficit of minerals in ruminants, a process for the production thereof as well as the use thereof. The present invention makes it possible to provide a potassium containing preparation or potassium bolus with a high content of up to 70 wt.-% of potassium salt, based on the total weight of the potassium containing preparation, by adding only a relatively small amount of calcium chloride (15 to 40 wt.-%) and water (10 to 22 wt.-%) to the potassium salt.

Described are strains including Enterococcus faecium strain 8G-1 (NRRL B-50173), Enterococcus faecium strain 8G-73 (NRRL B-50172), Bacillus pumilus strain 8G-134 (NRRL B-50174) and strains having all of the identifying characteristics of each of these strains. One or more of the strains can be used to reduce negative energy balance in a ruminant. They can also be used to improve other measures of ruminant health and/or performance. Methods of using the strains, alone and in combination, are described. Methods of making the strains are also provided.

Stable solid compositions have a first carrier and an adsorbate having at least one active component, such as, a vitamin D derivative and a hydrophobic stabilizer thereof, wherein the first carrier is a calcium phosphate or derivatives thereof having a solubility in water lower than 0.1 wt % at room temperature.

Stable solid compositions have a first carrier and an adsorbate having at least one active component, such as, a vitamin D derivative and a hydrophobic stabilizer thereof, wherein the first carrier is a calcium phosphate or derivatives thereof having a solubility in water lower than 0.1 wt % at room temperature.

A wildlife supplement and a related method of controlling supplement consumption, reducing mold in a supplement and/or administering a therapeutic agent, with a particular level of sodium, to an animal in the wild at a remote location is provided. The feed supplement can include sodium in an amount of 5% to 80% by weight, and optionally a feed ingredient, attractant, and/or a mineral supplement. The feed supplement can be applied in a remote area, optionally distal from any livestock feeding area. Consumption of the feed supplement by the animal can be self-regulated by the animal via the sodium content. Mold and/or bacterial growth can be limited via the sodium content. The sodium content can entice animals to consume a therapeutic agent in a controlled manner to orally self-vaccinate, treat and/or prevent a disease or condition.

A wildlife supplement and a related method of controlling supplement consumption, reducing mold in a supplement and/or administering a therapeutic agent, with a particular level of sodium, to an animal in the wild at a remote location is provided. The feed supplement can include sodium in an amount of 5% to 80% by weight, and optionally a feed ingredient, attractant, and/or a mineral supplement. The feed supplement can be applied in a remote area, optionally distal from any livestock feeding area. Consumption of the feed supplement by the animal can be self-regulated by the animal via the sodium content. Mold and/or bacterial growth can be limited via the sodium content. The sodium content can entice animals to consume a therapeutic agent in a controlled manner to orally self-vaccinate, treat and/or prevent a disease or condition.

The application relates to a preparation method of liquid bio-fermented feed, liquid bio-fermented feed and bio-fermented emulsion liquid feed, belonging to the technical field of feed preparation. The preparation method of the present application may overcome physiological problems of weaned piglets such as insufficient gastric acid secretion, low content and activity of digestive enzymes, incomplete development of immune system, disorder of intestinal microecosystem, and the like. The prepared bio-fermented liquid feed has good palatability and high nutrition, easy digestion, low acidity, and is a liquid feed similar to breast milk, which can both effectively alleviate the weaning stress syndrome of piglets, and effect a seamless transition from breast milk to conventional solid feed.