A method for separating of at least two transition metals, the method comprising: injecting a feed solution into a chromatography column comprising a chromatographic support media, the feed solution comprising at least two transition metals; eluting the feed from the column in an elution cycle by flowing an eluent through the column, wherein a concentration of the eluent is reduced during the elution cycle prior to elution of at least one of the transition metals.

A device (100, 100, 100) for separating an analyte (200) from other components in an electrolytic solution. The device comprises a housing (114, 115, 116, 117, 118, 119) provided with a solution inlet (104) and a solution outlet (105); a working electrode (101) arranged in the housing such that an electrolytic solution arranged to flow (F) from the inlet to the outlet contacts at least a portion of the working electrode; a counter electrode (102) arranged in the housing (114, 115, 116, 117, 118, 119). At least a portion of a surface of the working electrode (101) is provided with a polyelectrolytic coating (111), the polyelectrolytic coating (111) being arranged to upon application of a potential difference between the working electrode (101) and the counter electrode (102) switch between a first and second state, wherein in the first state an analyte (200) is captured in the polyelectrolytic coating (111) and in the second state a captured analyte (200) is released from the polyelectrolytic coating (111).

Herein is reported a method for determining the presence of antibody-Fab-FcRn interaction in an antibody-Fc-FcRn complex influencing the in vivo half-life comprising the steps of a) determining the retention time of the antibody on an FcRn affinity chromatography column with a positive linear pH gradient elution in the presence of a first sodium chloride concentration, and b) determining the retention time of the antibody on an FcRn affinity chromatography column with a positive linear pH gradient elution in the presence of a second sodium chloride concentration, whereby the presence of antibody-Fab-FcRn interaction in an antibody-Fc-FcRn complex influencing the in vivo half-life is determined if the retention time determined in step a) and the retention time determined in step b) are substantially different.

Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.

Apparatus for purifying a liquid comprising a target substance comprising at least two units arranged in series such that the feed stream of the second and any subsequent units comprises the product stream from a downstream unit, wherein each unit comprises specified components (i) to (vi), including a a switchable bypass assembly. Also claimed are the units and a flowpath assembly. The units may be essentially the same except for a device they contain, leading to advantages in terms of simplicity, cost and ease of operation, lower risk of operator error, easier maintenance and lower inventory of spare parts.