Methods of preparing highly purified steviol glycosides, particularly Rebaudioside D, are described. The methods include purification from the extraction stage of the Stevia rebaudiana Bertoni plant, purification of steviol glycoside mixtures, Rebaudioside D and Rebaudioside A from a commercial Stevia extract, and purification of Rebaudioside D from remaining solutions obtained after isolation and purification of Rebaudioside A and a high purity mixture of steviol glycosides. The methods are useful for producing high purity Rebaudioside D, Rebaudioside A, and steviol glycoside mixtures. The high purity steviol glycosides are useful as non-caloric sweeteners in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

The invention relates to a method for purifying a solution, the method comprising the following successive steps: bringing a solution to be purified into contact with an ion exchange resin by suspending the ion exchange resin in the solution to be purified, the ion exchange resin having the form of particles having a size Dv50 smaller than or equal to 200 m; separating the solution into a purified solution and a loaded resin; regenerating the loaded resin by passing at least one regenerating solution through a compact bed of loaded resin. The invention also relates to an assembly for implementing the method.

Automated two step chromatography purification system comprising a, system controller, a capture flow path comprising at least one pump, an elution flow path comprising at least one pump, and a valve arrangement for selective connection of two capture columns to the capture flow path and the elution flow path respectively such that both flow paths may be operated simultaneously and in parallel.

The present invention relates to a method for purifying a feed comprising a concentration of a target product in a chromatography system (10) having a first adsorption purifying unit (13). The first adsorption purifying unit has a capacity for binding the target product and is configured to receive the feed from a first holding tank (12), receiving continuous feed from a bioreactor (11), and to provide the target product (14) at an outlet (13b). The method comprises: a) loading (S20) the first adsorption purifying unit (13) with a volume of feed provided from the first holding tank (12), the volume of feed comprising an amount of the at least one target product corresponding to less than, or equal to, the capacity for binding the target product in the first adsorption purifying unit, b) washing, eluting, cleaning and regenerating (S30) the first adsorption purifying unit (13) while filling the first holding tank (12) with feed, said first holding tank (12) having a volume of at least the amount of the feed provided by the bioreactor (11) during this step, and repeating step a) and b) for a predetermined number of cycles.

The invention discloses a method for packing a plurality of uniform chromatography columns, comprising the steps of: a) providing a plurality of chromatography columns; b) providing a plurality of chromatography resin aliquots; c) packing the chromatography resin aliquots in the chromatography columns to provide a plurality of packed chromatography columns; and d) subjecting the packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns.

Examples of the present disclosure describe systems and methods for detecting and mitigating stack pivoting exploits. In aspects, various checkpoints may be identified in software code. At each checkpoint, the current stack pointer, stack base, and stack limit for each mode of execution may be obtained. The current stack pointer for each mode of execution may be evaluated to determine whether the stack pointer falls within a stack range between the stack base and the stack limit of the respective mode of execution. When the stack pointer is determined to be outside of the expected stack range, a stack pivot exploit is detected and one or more remedial actions may be automatically performed.

Provided is a bioreactor arrangement for producing a biopolymer expressed by a cell and a continuous process for a capturing the biopolymer employing two chromatography units operated in series or independently.

A method for lithium adsorption in a carbonate- and/or sulfate-containing solution, comprising using an aluminum-based lithium adsorbent for adsorption of lithium ions in the carbonate- and/or sulfate-containing solution, after the adsorption is saturated, using a weakly acidic high-concentration salt solution to transform the adsorbent, desorbing the transformed adsorbent by means of a low-concentration salt solution or water, and entering the next cycle for operation.

A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

An apparatus and method for carrying out continuous true moving bed chromatography using strong magnetic fields. More particularly the invention enables counter-flow moving bed chromatography with much better efficiency than batch chromatography; and with design and operation much simpler than simulated moving bed chromatography.