The disclosure provides methods for the isolation, separation, and purification of antibodies. The method comprises an affinity chromatography capture step, anion exchange chromatography polishing step, and cation exchange chromatography polishing step.

The disclosure provides methods for the isolation, separation, and purification of antibodies. The method comprises an affinity chromatography capture step, anion exchange chromatography polishing step, and cation exchange chromatography polishing step.

Provided is a method for producing a lithium-containing solution that allows increasing a content rate of lithium in a solution after an eluting step, and suppressing an amount of an eluted solution used in a process after the eluting step, thus suppressing production cost of lithium.

A method for producing a lithium-containing solution includes an adsorption step of bringing a lithium adsorbent obtained from lithium manganese oxide in contact with a low lithium-containing solution to obtain post-adsorption lithium manganese oxide, an eluting step of bringing the post-adsorption lithium manganese oxide in contact with an acid-containing solution to obtain an eluted solution, and a manganese oxidation step of oxidating manganese to obtain a lithium-containing solution with a suppressed manganese concentration. The adsorption step, the eluting step, and the manganese oxidation step are performed in this order, and the acid-containing solution includes the eluted solution with acid added. The method allows the usage amount of the acid in the eluting step to be suppressed, the content rate of lithium in the eluted solution after the eluting step to be increased, and thus the production cost of the lithium-containing solution to be suppressed.

A method is provided for manufacturing high-purity vitamin E compounds by selectively separating vitamin E compounds such as tocotrienols and tocopherols from oil raw materials such as deodorized distillate, and more specifically, a method is provided, applying to a rich fraction of vitamin E compounds, for obtaining high-purity vitamin E compounds by separating and removing only free fatty acids contained as impurities. The method comprises a process in which a solution containing vitamin E compounds and free fatty acids is placed in contact with a weakly basic anion exchanger, and the free fatty acids are preferentially adsorbed.

The present invention provides an apparatus and method for producing ultrapure water of extremely high purity that sufficiently meets the requirement for its quality at low production cost with reduced footprint. The apparatus for producing ultrapure water includes a pretreatment system, a primary water purification system, and a subsystem, wherein the primary water purification system includes a high-pressure reverse osmotic membrane separation unit, a degassing unit, an ultraviolet oxidation unit, and an ion-exchange unit in this order.

The present invention relates to a single module, flow-electrode apparatus for continuous water desalination, ion separation and selective ion removal and concentration by capacitive deionization, comprising: a first current collector (1), a first compartment (1′) for a flow electrode, a first ion exchange membrane (AEM, CEM), a first liquid-permeable channel (6a) next to the first ion exchange membrane (AEM, CEM), a second ion exchange membrane (CEM, AEM) with a fixed charge opposite to that of the first ion exchange membrane (AEM, CEM) next to the first liquid-permeable channel (6a), a second liquid-permeable channel (6b) next to the second ion exchange membrane (CEM, AEM), a third ion exchange membrane (AEM, CEM) having the same fixed charge as the first ion exchange membrane (AEM, CEM) next to the second liquid-permeable channel (6b), a second compartment (2′) for a flow electrode, and a second current collector (2), wherein a fluid (4) containing suspended conductive particles or a mixture of conductive and non-conductive particles or particles made of a mixture of conductive and non-conductive materials (5) is provided in the first and second compartments (1′, 2′), acting as the flow electrode, as well as a corresponding method.

A silicone polymer production method achieves a significantly improved yield due to the needlessness of a rinsing step and can produce a stable silicone polymer. The production method for a silicone polymer includes a step of preparing at least one silane compound selected from the compounds represented by general formula (1); R.sub.aSi(OR.sup.1).sub.4-a (1), wherein R is a hydrogen atom or a monovalent organic group, R.sup.1 is a monovalent organic group, and a is an integer of 1 or 2 and the compounds represented by general formula (2); Si(OR.sup.2).sub.4 (2), wherein R.sup.2 is a monovalent organic group, a step of hydrolyzing it with a quaternary ammonium compound in the presence of water, and a step of bringing it into contact with a cation exchange resin.

Disclosed is a chromatographic method for separating and purifying a recombinant human fibronectin from a genetically engineered rice seed that expresses the human fibronectin. In the method, the genetically engineered rice seed is milled, mixed with an extraction buffer, and then filtered to obtain a crude extract comprising the recombinant human fibronectin; the crude extract comprising the recombinant human fibronectin is subjected to cation exchange chromatography, so as to perform primary separation and purification, thereby obtaining a primary product comprising the recombinant human fibronectin; and the primary product is subjected to anion exchange chromatography so as to perform final separation and purification to obtain the recombinant human fibronectin as a target substance. The method is low cost and easily utilized on an industrial scale. The obtained OsrhFn target substance has a SEC-HPLC purity greater than 95% with excellent bioactivity.

The invention relates to an apparatus for applying foliar spray and more specifically, but not exclusively, to an apparatus for applying a carbon rich foliar spray. The apparatus (1) includes supply means (2) for supplying water (3) to the apparatus. The supply means (2) is in the form of a water tank (4) with pipe (5) extending from the tank, through a reverse osmosis water filtration unit (12), pump (6), and valve (7). A solute container (8) for holding solute (9) in the form an electrolyte solution of sodium ions (Na+) and bicarbonate ions (HCO3—) formed by mixing sodium bicarbonate and water (3) from the supply means (2). The combinatory part (10) is connected to an ion exchange part (15) through valve (19), pipe (17) and pump (18). The prepared solution is the product of passing the diluted electrolyte solution (11) through the ion exchange column (15). The prepared solution now contains mostly negatively charged bicarbonate anions (HCO3—) and may be applied to the foliage of plants (26) through pump (27), pipes (28), and finally micro sprayers (29).

Described herein is a method of adjusting the composition of a chromatography product to achieve a target enriched proportion of a desired component from an input feed having a lower proportion of the desired component using simulated moving bed (“SMB”) chromatography wherein the eluent for the SMB apparatus may comprise the very input feed being enriched. The method is exemplified by enriching a high fructose corn syrup from a 42% fructose syrup to a 55% fructose syrup without substantially reducing the dissolved solids concentration of the 55% syrup relative to the input 42% syrup. The 42% syrup is also used as the eluent for the SMB apparatus and may be reconstituted from the raffinate stream by passing the raffinate stream over a glucose isomerase column alone or in combination with a dextrose feed. The method reduces water usage and saves energy by minimizing the need for evaporation to obtain a 55% fructose syrup with a high dissolved solids content.