The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

The present invention relates to a method of preparing a population of antibodies, whereby a desired high-purity and high-quality population of antibodies can be prepared by removing impurities without using an expensive protein A column, and in particular, production costs can be significantly reduced while achieving process automation; and a population of antibodies prepared thereby.

The present invention relates to a method of preparing a population of antibodies, whereby a desired high-purity and high-quality population of antibodies can be prepared by removing impurities without using an expensive protein A column, and in particular, production costs can be significantly reduced while achieving process automation; and a population of antibodies prepared thereby.

The disclosure relates to a method for separation and purification of N-acetyl-glucosamine, and belongs to the technical field of biological engineering. In the disclosure, a raw material solution containing N-acetyl-glucosamine is obtained by microbial fermentation or by hydrolyzing the chitin. The raw material solution is subjected to flocculation pretreatment, and continuous centrifugation or pressure filtration is performed to remove suspended solids such as microorganisms, proteins and polysaccharides to obtain clear liquid. Double-stage ion exchange chromatography is performed to remove impurities such as charged organic molecules and inorganic salts. Membrane concentration is performed to efficiently remove water to improve the concentration of the target product. Spray drying or further evaporation concentration and crystallization are performed. Finally drying is performed to obtain an N-acetyl-glucosamine crystal of which the purity is more than 99%.

A method for producing 225.sup.A including: a method (X) for purifying a .sup.226Ra-containing solution, including an adsorption step of allowing a .sup.226Ra ion to adsorb onto a carrier having a function of selectively adsorbing a divalent cation by bringing a .sup.226Ra-containing solution into contact with the carrier under an alkaline condition, and an elution step of eluting the .sup.226Ra ion from the carrier under an acidic condition; a method for producing a .sup.226Ra target, including an electrodeposition liquid preparation step of preparing an electrodeposition liquid by using a purified .sup.226Ra-containing solution obtained by the method (X), and an electrodeposition step of electrodepositing a .sup.226Ra-containing substance on a substrate by using the electrodeposition liquid; and a step of irradiating a .sup.226Ra target produced by the method for producing a .sup.226Ra target with at least one selected from a charged particle, a photon, and a neutron by using an accelerator.

The present disclosure provides a system for efficiently preparing taurine, including: a solution storage unit configured to store a solution containing alkali metal taurinate, the solution being prepared by an ethylene oxide process; an ion exchange unit including at least one ion exchange resin column each configured to be activated by a first activation manner or a second activation manner independently, the first activation manner using sulfurous acid for activation to obtain alkali metal bisulfate and taurine, and the second activation manner using sulfuric acid for activation to obtain alkali metal sulfate and taurine; and a dispensing unit connected to the solution storage unit and the ion exchange unit respectively, and configured to adjust an amount of a solution conveyed from the solution storage unit to each of the at least one ion exchange resin column in the ion exchange unit.

A method of recovering a sugar by separating a fermentation inhibitor and the sugar from a sugar solution containing the fermentation inhibitor, the method including: bringing the sugar solution containing the fermentation inhibitor into contact with a basic anion exchange resin filled into a column, followed by separation of the fermentation inhibitor and the sugar by a difference in retention time therebetween through use of water as an eluent, and separate recovery of a fraction containing the fermentation inhibitor and a fraction containing the sugar, wherein the basic anion exchange resin is previously treated with a solution containing the fermentation inhibitor.

Disclosed is a natural compound sweetener, comprising mogroside V, rebaudioside A, natural tea theanine and dietary fibre. The method for preparing the sweetener comprises the steps of: (1) dissolution, filtration, concentration and sterilization: dissolving the mogroside V, rebaudioside A, natural tea theanine and dietary fibre in water, filtering, concentrating in a vacuum, and sterilizing to obtain a sterilized solution; and (2) paste-collection, drying and granulation: carrying out paste-collection on the sterilized solution obtained in the step (1), vacuum drying the collected liquid paste, and drying and then granulating the dry powder to obtain the sweetener.

Provided herein are methods of separating host cell lipases from an anti-LAG3 antibody or antigen binding fragment in chromatographic processes and methods of improving polysorbate-80 stability in an anti-LAG3 antibody formulation by separating host cell lipases from the anti-LAG3 antibody or antigen binding fragment using chromatographic processes. Also provided are pharmaceutical compositions comprising an anti-LAG3 antibody or antigen binding fragment and less than 2 ppm of a host cell lipase.