Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

Disclosed are purification systems and methods for providing purified preparations of antibodies from a fluid, particularly a biological fluid comprising or suspected to contain antibody (e.g., blood, serum, plasma, ascites fluid). Reusable and stable synthetic purification columns comprising membranes of a suitable separation matrix material, such as a nylon membrane or regenerated cellulose membrane, having conjugated thereto a small molecule capture ligand, such as a short peptide or protein capable of acting as a ligand for a particular antibody of interest, such as a peptide having a sequence with binding affinity for a nucleotide binding site (NBS) of a selected antibody of interest, are also provided. Methods of preparing the purification columns are also disclosed. Methods for preparing high yield and high purity therapeutic antibody preparations, such as anti-cancer therapeutics, from a biological fluid, are also presented.

Provided herein are methods and compositions for nucleic acid processing comprising obtaining a stabilized sample comprising a nucleic acid molecule complexed to at least one nucleic acid binding protein; contacting said stabilized sample to a dendrimer comprising a plurality of nucleic acid binding moieties such that said nucleic acid molecule forms a complex with said plurality of nucleic acid binding moieties; contacting said complex to an endonuclease to cleave said nucleic acid molecule between contact points of said nucleic acid molecule and said plurality of nucleic acid binding moieties creating a plurality of fragments of said nucleic acid molecule each complexed with a nucleic acid binding moiety of said plurality of said nucleic acid binding moieties; isolating said product using an agent that binds to said dendrimer; joining said plurality of fragments to each other to create a concatemer comprising each of said plurality of fragments of said nucleic acid molecule; and isolating said concatemer from said dendrimer.

In order to provide a separating agent for liquid chromatography that is able to separate a protein using target characteristics as an index while retaining the original steric structure, the separating agent for liquid chromatography is equipped with a substrate, a recognition site including a compound that operates by recognizing characteristics of biopolymers such as proteins, and a spacer that bonds the recognition site to the substrate, wherein the spacer has an effective length to enable the recognition site to operate by reaching deep portions of the steric structure of a target biopolymer.

The invention relates to a method for determining the presence of at least one distinct polypeptide in a biological sample comprising contacting the biological sample with a hydrolyzing agent, wherein the hydrolyzing agent is capable of hydrolyzing the distinct polypeptide in a sequence-specific manner such that at least one distinct peptide having a predetermined peptide measured accurate mass would result if the at least one distinct polypeptide were present in the biological sample, to obtain a hydrolyzed sample; bringing the hydrolyzed sample in contact with a substrate comprising at least one immobilized binding partner, wherein the at least one immobilized binding partner is capable of specifically binding the distinct peptide; removing the hydrolyzed sample from the substrate in a manner such that the distinct peptide would remain bound to the immobilized binding partner; contacting the substrate with an elution solution, wherein the distinct peptide would dissociate from the immobilized binding partner into the elution solution; subjecting a portion of the elution solution to liquid chromatography to segregate a plurality of molecules in the portion of the elution solution to obtain sorted molecules; determining the measured accurate mass of at least one sorted molecule present in the elution solution; and determining the presence of the at least one distinct polypeptide in the biological sample when a measured accurate mass of at least one molecule is substantially equal to the predetermined peptide measured accurate mass.

The present disclosure provides immunoprecipitation (IP) methods such as antibody-mediated techniques using a bridged tandem antibody complex. In other aspects, the present disclosure also provides compositions, kits, and systems useful for use in the immunoprecipitation methods described herein.

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.

Aspects of the present disclosure include a solid phase sorbent for preparation of analytical samples. The solid phase sorbent includes particles that are surface modified with an -cyclodextrin moiety. Also provided is a method of reducing matrix effects in an analytical sample. In some embodiments, the method includes contacting a sample comprising a matrix-interfering agent and an analyte with -cyclodextrin modified particles to produce a contacted sample wherein the matrix-interfering agent binds to the -cyclodextrin modified particles; separating the -cyclodextrin modified particles from the contacted sample to produce a matrix-reduced composition; and detecting the analyte in the matrix-reduced composition. Systems for practicing the subject methods are provided that include the subject solid phase sorbent.

The present invention discloses a novel method of isolating pure bakuchiol, substantially free of furanocoumarins (psoralen and isopsoralen) from the seeds of Psoralea sp using a combination of processes that include supercritical fluid extraction, high vacuum distillation and column purification. The invention also disclose compositions comprising bakuchiol that is free of furanocoumarins.

The present invention relates to the chromatographic isolation of a target cell or another complex biological material, in particular by column chromatography such as affinity chromatography or gel permeation chromatography. The invention employs a receptor binding reagent that binds to a receptor molecule that is located on the surface of a target cell. The invention in general provides novel methods for the traceless isolation of biologic materials such as cells, cell organelles, viruses and the like. The invention also relates to an apparatus for the isolation of cells and other complex biological materials.