The invention relates to a method for separation of biopolymer molecules, particularly biopolymer molecules from the group consisting of mono- a multi-phosphorylated peptides, recombinant peptides/proteins with a polyhistidine tag (His-tag) or with another chemically similar biospecific tag, cysteine-containing peptides/proteins and nucleic acids, in which a biopolymer molecule is bound in a binding solution by a specific binding to a carrier, which contains a core with dimensions in nano- and/or submicro- and/or microscale, which is composed of oxide of at least one transition metal and/or silicon oxide, on whose surface is deposited at least one continuous or non-continuous layer and/or nanoparticles of magnetic metal oxide and/or such nanoparticles are deposited in its inner structure, and subsequently undesirable and non-specifically bound components are washed off at least once from the carrier-bound bio-molecules by a washing solution, whereupon biopolymer molecules are eluted from it by changing pH and/or by using an elution solution. The invention also relates to a carrier for application of this method.

A stationary phase for solid-phase microextraction (SPME) devices is based on nickel and titanium alloy nuclei and a metal-organic framework (MOF) exterior, which may be used for chromatographic analysis in environmental, food, etc. applications. The method of preparation of the stationary phases includes a number of steps which provide a covalent adhesion of the MOF to the nickel/titanium alloy. In these stationary phases, the metal-organic framework is the only component that comes into contact with the sample to be analysed. The interior of the stationary phase is executed in nitinol and endows the system with thermal and mechanical stability this being the first time that it is used to support a metal-organic framework, and presenting extractive advantages in comparison with commercial SPME stationary phases.

A coating of a random copolymer of acrylamide and a second monomer, e.g. glycidoxylmethacrylate, for a silica surface is described. The coating is applied to chromatographic support structures having silica based surfaces. The coating is functionalized to produce protein chromatography matrices that are particularly useful for extracting trace amounts of biomarker molecules from biological samples.

A method for reacting a reaction object with a liquid containing the reaction object in contact with a granular porous body. The upper limit D (mm) of the particle diameter of the granular porous body is determined from D=0.556×LN (T)+0.166 in a column flow method in non-circulation type, and determined from D=0.0315×T+0.470 in the column flow method in a circulation type and a shaking method. The granular porous body includes a skeleton body including an inorganic compound having a three-dimensional continuous network structure, and has a two-step hierarchical porous structure including through-holes formed in voids in the skeleton body, and pores extending from a surface to an inside of the skeleton body and dispersed on the surface. A functional group having affinity with the metal ion is chemically modified on a surface of the granular porous body.

The present invention relates to a method of controlled competitive exchange of a first agent bound to a metal coordination complex with a competing agent, with selection of the nature of the first agent allowing a tailoring of the binding strength and thereby allowing for a desired level of control for subsequent displacement by the selected competing agent. The method may be employed for release of therapeutic agents, sequestration of larger molecules from a sample, generation of a preferred binding surface and the like.

The invention relates to the isolation or extraction of exosomes.

A method for producing an aqueous solution of purified orthoperiodic acid with a reduced Cr content; a method for producing a semiconductor device that includes etching a Ru layer on a semiconductor substrate with an etchant obtained by the method; and an aqueous solution of orthoperiodic acid with a reduced Cr content. The method includes bringing an aqueous solution of crude orthoperiodic acid into contact with a metal removing agent including a chelating resin, the aqueous solution of crude orthoperiodic acid containing orthoperiodic acid and water and having an orthoperiodic acid content of 15% by mass or less and a Cr content of 1 ppb by mass or more based on the total mass of the aqueous solution of crude orthoperiodic acid.

Extraction of platinum-group elements, e.g. Pd, by adsorption from acidic aqueous solutions, using chelating acrylic fibers having amidoxime substituents followed by recovery by elution with an HCl-thiourea solution. From about 10% to 100% of the acrylic fiber CN are converted to amidoxime by reaction with NH.sub.2OH (hydroxylamine) in H.sub.2O/MeOH solution in the range of 30° C.-90° C. for from 15 min to 72 hrs. The adsorptive loading of elements onto the fiber and the efficiency of elution therefrom is substantially 100%, in multiple cycles of adsorption/elution. The novel fiber/extraction process is rapid, lending it to a continuous recovery operation. A portion of the CN groups of may be converted to carboxylate groups by reaction with NaOH. Short lengths of fiber are loaded into a vertical column and the pregnant solution introduced. Upon breakthrough, the fibers may be eluted, washed and recycled hundreds of times without removal from the column.

A method for recovering an acidic gas, includes: a step of bringing a gas to be treated that contains an acidic gas into gas-liquid into contact with an amine absorbing solution, allowing the amine absorbing solution to absorb the acidic gas, thereby removing the acidic gas from the gas to be treated; a step of allowing the amine absorbing solution that has absorbed the acidic gas to release the acidic gas, thereby regenerating the amine absorbing solution, and at the same time, recovering the released acidic gas; and an analysis step of calculating concentrations of iron ions and/or heavy metal ions in the amine absorbing solution.

The present invention provides an in vitro method for concentrating infectious pathogens found in a biological sample obtained from an individual who is suspected of being infected with the pathogens. Provided herein is also an in vitro method for reducing or eliminating blood cells from a sample obtained from an individual suspected to being infected with an infectious pathogen. The present invention also provides a method for diagnosing malaria and a method for determining if an individual is infected with a pathogen. Provided herein is also a concentrator and a kit for use with the methods.