The invention relates to the isolation or extraction of exosomes.

Beads with functionalized material applied to them are exposed to an acoustic field to trap or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes.

An apparatus and method for recovering metal from a solution comprising a metal-selective sorbent disposed in a column. Additional embodiments provide for using a metal-selective membrane configured for selective transport, isolation, retention, and recovery of metal ions and compounds; electrodialysis and forward osmosis apparatuses to recover metal from a solution. A modular system to process a solution at a remote field site is disclosed. The process is a green process and produces limited to no industrial waste.

The present disclosure provides a method of separating Mo-99 from W-187 from a solution comprising Mo-99 and W-187. The method comprises contacting a tridentate diglycolanude ligand with a solution comprising Mo-99 and W-187 and eluting W-187 from the tridentate diglycolanude ligand to thereby an eluate comprising W-187.

Provided herein are affinity agents comprising ligands that specifically bind adeno-associated virus. The affinity agents are useful for binding, isolation, and/or purification of adeno-associated virus. Further disclosed are amino acid sequences of binding motifs or polypeptides comprised by the ligands, and associated modifications of the binding motifs and/or polypeptides, as well as a method of making the affinity agents.

The present disclosure relates to methods of re-oxidizing an antigen-binding protein.

The present invention provides a stationary phase for solid-phase microextraction (SPME) devices based on nickel and titanium alloy nuclei and a metal-organic framework (MOF) exterior, which may be used for chromatographic analysis in environmental, food, etc. applications. The method of preparation of the stationary phases consists of a number of steps which provide a covalent adhesion of the MOF to the nickel/titanium alloy. In these stationary phases, the metal-organic framework is the only component that comes into contact with the sample to be analysed. The interior of the stationary phase is executed in nitinol and endows the system with thermal and mechanical stability, this being the first time that it is used to support a metal-organic framework, and presenting extractive advantages in comparison with commercial SPME stationary phases.

A method of collecting a nucleic acid from a sample containing a nucleic acid using a support of aluminum oxide having a surface where a water-soluble neutral polymer is adsorbed, the method including steps a to c: step a: a step of bringing the support into contact with the sample containing a nucleic acid to adsorb the nucleic acid on the support; step b: a step of bringing the support on which the nucleic acid is adsorbed into contact with a solution A containing 1 mM or more and 40 mM or less of a chelating agent; and step c: after the step b, a step of bringing the support on which the nucleic acid is adsorbed into contact with a solution B containing 50 mM or more of a chelating agent to elute the nucleic acid.

The present disclosure is directed to methods of characterizing a system containing a chromatographic column. The methods can include introducing a sample comprising a positive control and a negative control to the system containing a chromatographic column, wherein the positive control is a sensitive probe that interacts with the system and the negative control is substantially non-interacting with the system; after passing the sample through the chromatographic column, detecting the positive control and the negative control; and determining system suitability by comparing the amount of detected positive control to negative control. In some embodiments, determining system suitability (e.g., inertness of sample to the system) is accomplished by determining a ratio of detected positive control to negative control.

A method for reacting a reaction object with a liquid containing the reaction object in contact with a granular porous body. The upper limit D (mm) of the particle diameter of the granular porous body is determined from D=0.556×LN (T)+0.166 in a column flow method in non-circulation type, and determined from D=0.0315×T+0.470 in the column flow method in a circulation type and a shaking method. The granular porous body includes a skeleton body including an inorganic compound having a three-dimensional continuous network structure, and has a two-step hierarchical porous structure including through-holes formed in voids in the skeleton body, and pores extending from a surface to an inside of the skeleton body and dispersed on the surface. A functional group having affinity with the metal ion is chemically modified on a surface of the granular porous body.