A sequential mixer for mixing a heated hydrocarbon stream and a supercritical fluid to produce an intimately mixed stream upstream of a supercritical reactor. The sequential mixer comprising a body having a body length and a body diameter; a hydrocarbon inlet physically connected to the body, having an inlet diameter, the heated hydrocarbon stream is introduced through the hydrocarbon inlet; a mixed stream outlet physically connected to the body and fluidly connected to the supercritical reactor, having an outlet diameter; a traversing axis extending through the center of the body from the hydrocarbon inlet to the mixed stream outlet; and a plurality of fluid ports physically connected to the body, the plurality of fluid ports are arranged in a port alignment arrayed along the traversing axis, each fluid port has a port diameter and a port angle, the supercritical fluid is injected through the plurality of fluid ports.

A method for the continuous flow synthesis of (R)-4-halo-3-hydroxy-butyrate using a micro-reaction system. The micro-reaction system includes a micro-mixer, a certain number of micro-reaction units that are successively connected in series, a pH regulating system and a back pressure valve. The micro-reaction unit is composed of a micro-channel reactor and a pH regulator that are sequentially connected with each other. A substrate solution containing halogenated acetoacetate and a biocatalyst solution are simultaneously pumped into the micro-reaction system to enable continuous flow biocatalytic asymmetric reduction reaction of the halogenated acetoacetate to obtain the target product (R)-4-halo-3-hydroxy-butyrate.

A microfluidic chip, device, system, the use thereof and method for the generation of aqueous droplets in emulsion oil for nucleic acid amplification.

Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.

Disclosed is an emulsion production microfluidic device which includes: a first channel, including an entry port configured to inject a phase to be dispersed, a second channel, including an entry port configured to inject a continuous phase and an emulsion exit port, and at least one array of microchannels, a height of each of the microchannels being smaller than a height of the first channel; the second channel includes a first part connected to the outlet of each microchannel and at least a second part along the first part, the first part being between the array of microchannels and the second part, the first part having a height greater that the height of each microchannel, and the second part having a height greater than the height of the first part.

An embodiment for a microfluidic device is provided. The device comprises two areas, arranged side-by-side, and a trigger channel. They include a first area, which is delimited by a first liquid pinning barrier, and a second area, which is delimited by a second liquid pinning barrier. The latter extends parallel to the first liquid pinning barrier to delimit a corridor. The trigger channel extends through the corridor between the two areas. In addition, the trigger channel connects the first liquid pinning barrier with the second liquid pinning barrier, allowing a first liquid pinned at the first liquid pinning barrier and a second liquid pinned at the second liquid pinning barrier to be contacted, each, by a reverse flow of the second liquid in the trigger channel and thereby start mixing at a level of the corridor, in operation. The invention is further directed to related methods of operation.

A device for gassing a liquid, including a rotor, which is driven to rotate and has multiple vanes for conveying the liquid, and a stator, which surrounds the rotor and has a plurality of flow channels, which each extends starting from a radially inner inlet opening adjacent to the rotor through the stator to a radially outer outlet opening and are delimited along their length by side walls, bottom surfaces, and top surfaces and can be acted on with liquid by the rotor in the region of the inlet opening, wherein between the inlet opening and the outlet opening, the side walls and/or bottom and top surfaces of the flow channels have a multitude of gassing openings, which can be acted on with compressed gas from a compressed gas source in order to introduce this gas into the flow channels. A corresponding method for gassing a liquid is also disclosed.

The invention relates to an apparatus for purification of biomolecules, comprising an injection device comprising an injection channel provided with at least one inlet for a precipitation agent composition and a plurality of outlets on the external surface of the injection channel and a mixing device comprising a mixing channel with at least one main inlet for a composition to be purified and at least one main outlet, and a plurality of secondary inlets, wherein the injection device and the mixing device are coupled such that each of the plurality of outlets of the injection device is connected to a respective secondary inlet of the mixing channel. The invention further relates to a set comprising the apparatus, a composition comprising at least one biomolecule and at least one impurity, and a composition comprising at least one precipitation agent, and to a method for purification of biomolecules using the apparatus.

A nozzle for injecting a first liquid mass into a stream of second liquid mass flowing within a pipe, comprising a first, outer, cylinder and second, inner, cylinder concentrically arranged about a nozzle axis, securing means for securing the nozzle to a wall of the pipe with the nozzle axis orthogonal to the pipe wall, the nozzle projecting into an interior of the pipe in use, duct means for receiving the first liquid mass and transporting it to the interior of the inner and outer cylinders, the inner cylinder comprising at least one hole arranged to expel liquid therethrough, and the outer cylinder comprising at least one hole arranged to expel liquid therethrough.

A nozzle for injecting a first liquid mass into a stream of second liquid mass flowing within a pipe, comprising a first, outer, cylinder and second, inner, cylinder concentrically arranged about a nozzle axis, securing means for securing the nozzle to a wall of the pipe with the nozzle axis orthogonal to the pipe wall, the nozzle projecting into an interior of the pipe in use, duct means for receiving the first liquid mass and transporting it to the interior of the inner and outer cylinders, the inner cylinder comprising at least one hole arranged to expel liquid therethrough, and the outer cylinder comprising at least one hole arranged to expel liquid therethrough.