Microfluidic devices for use with reagents bound to microspheres for determination of the concentration of an analyte in a liquid sample are provided. The devices include two sequential mixing channels that promote rapid binding of microsphere-bound reagents with reagents in solution and a means for detecting labeled microsphere-bound reaction products. Also provided are methods for using the devices with microsphere-bound reagents to determine the concentration of an analyte in a liquid sample and to measure the binding affinity of antibody for an antigen.

Disclosed herein are methods and devices for antigen-specific T cell identification or neoantigen identification. Also disclosed herein are devices for separating and isolating antigen- specific T cells or other particles of a certain size from a population of particles of different sizes. Also describe herein are methods and devices for the separation and isolation of barcoded T cells from other nanoparticles containing barcodes for subsequent analysis and further processing of a viable T cell.

An apparatus or method for generating a microfoam, the apparatus comprising a channel having an inlet and an outlet, a source of foamable liquid and pressurised gas arranged to feed into the inlet, wherein the channel is comprised of a spatially oscillating flow channel to provide an oscillating flow direction, the spatially oscillating flow channel oscillating about a bulk flow direction, the spatially oscillating flow channel providing a sequence of planar cross-sections perpendicular to the flow direction, with a sub-sequence of planar cross-sections that are perpendicular to the bulk flow direction in the plane in question, the sub-sequence comprising at least one plane that does not overlap with at least one other plane in the sub-sequence.

A fluidic device for mixing a reagent fluid with a fluid sample comprises a supply channel having a reagent inlet, a sample inlet and a first reagent storage, coupled to the supply channel; a mixer for mixing the reagent with the fluid sample, having a mixer inlet coupled to the supply channel at a position in between the sample inlet and the first reagent storage; In a first stage, when the reagent fluid is supplied in the reagent inlet, the reagent is provided in the supply channel and the first reagent storage, and such that the reagent is thereafter stationed in the supply channel and the first reagent storage until a fluid sample is provided in the sample inlet. When the fluid sample is supplied in the sample inlet, the supplied fluid sample and the stationed reagent flows into the mixer thereby mixing both fluids.

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

A mixing device for mixing exhaust gas from a combustion engine of a vehicle with a solution for lowering the content of nitrogen oxides in the exhaust gas includes a casing with an exhaust inlet, an exhaust outlet, and a corner region in which an exhaust gas flow is diverted toward the exhaust outlet. The corner region has an opening for a dosing unit. A flow guide is arranged within the casing. The flow guide is configured to divide a total of the exhaust gas flow into a first exhaust gas stream and a second exhaust gas stream. A gap for the second exhaust gas stream is formed between the flow guide and a side wall of the casing. In the side wall the opening for the dosing unit is located. An end region of the flow guide is arranged upstream of the opening for the dosing unit.

In a method of detecting a test substance, a test substance is detected using a sample analysis cartridge supplied with a sample. The sample analysis cartridge includes: a passage part having a gas-phase space; and liquid containers communicating with the passage part through openings. The liquid containers include: a first liquid container containing a first liquid containing magnetic particles; and a second liquid container containing a second liquid containing a labeled substance. The magnetic particles are sequentially transported to the liquid containers through the gas-phase space in the passage part. Thus, the magnetic particles carry a complex of the test substance and the labeled substance. The test substance is detected based on the labeled substance in the complex.

A system, device, and method for receiving, preparing and identifying a biological sample. Some parts may be user held and actuated. The sample may be detected by an array of sensors. The system may comprise reusable and disposable components arranged to be connected together. The biological sample may contain nucleic acids, proteins, or other molecules to be detected.

One or more homogenizing elements are employed in a flow through, multi-detector optical measurement system. The homogenizing elements correct for problems common to multi-detector flow-through systems such as peak tailing and non-uniform sample profile within the measurement cell. The homogenizing elements include coiled inlet tubing, a flow distributor near the inlet of the cell, and a flow distributor at the outlet of the cell. This homogenization of the sample mimics plug flow within the measurement cell and enables each detector to view the same sample composition in each individual corresponding viewed sample volume. This system is particularly beneficial when performing multiangle light scattering (MALS) measurements of narrow chromatographic peaks such as those produced by ultra-high pressure liquid chromatography (UHPLC).

Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.