The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

One or more homogenizing elements are employed in a flow through, multi-detector optical measurement system. The homogenizing elements correct for problems common to multi-detector flow-through systems such as peak tailing and non-uniform sample profile within the measurement cell. The homogenizing elements include coiled inlet tubing, a flow distributor near the inlet of the cell, and a flow distributor at the outlet of the cell. This homogenization of the sample mimics plug flow within the measurement cell and enables each detector to view the same sample composition in each individual corresponding viewed sample volume. This system is particularly beneficial when performing multiangle light scattering (MALS) measurements of narrow chromatographic peaks such as those produced by ultra-high pressure liquid chromatography (UHPLC).

The present invention relates to a tubular mixing device (6) with reversed coiled configuration (also referred to as “coiled flow reverser” CFR)) wherein after a number n of turns (2) with n=1; 2 the flow path is reverted to the opposite direction, and wherein the device (6) has an overall straight coil axis.

Disclosed herein are methods and devices for antigen-specific T cell identification or neoantigen identification. Also disclosed herein are devices for separating and isolating antigen-specific T cells or other particles of a certain size from a population of particles of different sizes. Also describe herein are methods and devices for the separation and isolation of barcoded T cells from other nanoparticles containing barcodes for subsequent analysis and further processing of a viable T cell.

A medicament preparation system includes a disposable cartridge with a flow path. Various sensors may be placed on the cartridge to measure qualities of the fluid flowing through the flow path. The sensors are placed in precise locations using various approaches that make manufacturing of the cartridge efficient and repeatable. A drain line that is susceptible to fouling may be preattached and various approaches are used to remove or reduce the fouling. An elastomeric contact can also be present in the medical preparation system and used in a conductivity measurement subsystem.

The present disclosure relates to a fluid path member for generating nano-bubbles, and a fluid path integrator and a nano-bubble generator that use the same. The fluid path member may be configured such that a perimeter length of a cross-section of a fluid path is greater than a cross-sectional area of the fluid path so as to maximize a friction area per volume of fluid. In addition, the fluid path member may be configured such that a single fluid path is continuously formed over several tens of meters or more without a joint. Further, the fluid path member may be configured with a high density. Accordingly, the fluid path member may have improved ability to generate the nano-bubbles. A fluid path member configured to generate nano-bubbles according to some embodiments of the present disclosure includes a body formed as a bendable single tube, wherein the body is configured such that one or more dividing walls dividing a fluid path space inside a fluid path so as to expand a surface area and a friction area of a fluid are continuously integrally formed along a flow direction of the fluid, wherein the body is formed of a soft material of any one of silicone, rubber, and soft resin material so as to be freely bent and wound, and wherein the body is manufactured by extrusion molding such that the one or more dividing walls are continuously formed in a longitudinal direction of the body.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.

A method and a system are described for mixing liquid chemicals at dynamically changing or static ratios during a given dispense, with extremely high uniformity and repeatability. A mixer includes multiple fluid supply lines including elongate bladders defining a linear flow path and being configured to laterally expand to collect a process fluid and laterally contract to deliver a selected volume of the process fluid to the mixer.

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.