A flow conditioning device for conditioning a wet gas stream having a plurality of liquid droplets and a gas flow is presented. The flow conditioning device includes a first segment including a first convergent section configured to break the plurality of liquid droplets from a first size to a second size. Further, the flow conditioning device includes a second segment coupled to the first segment and including a second convergent section configured to break the plurality of liquid droplets from the second size to a third size.

A fire-fighting system including: a pressurized gas supply; a vessel for storing a solution of water and a foaming agent, the vessel being connected to the pressurized gas supply for pressurizing the vessel in use; and a mixer for mixing gas from the pressurized gas supply with the solution to generate foam, wherein the mixer includes: a solution inlet for receiving the solution from the vessel; a foam outlet; one or more injection ports connected to the pressurized gas supply for injecting gas bubbles into the solution to form a mixture of the solution and the gas bubbles; and a foaming chamber extending between the solution inlet and the foam outlet, the mixture flowing through the foaming chamber to generate foam at the foam outlet, at least one internal surface of the foaming chamber including a shearing structure for shearing the gas bubbles as the mixture flows through the foaming chamber.

Ancillary embodiments and modifications to a homogenizer unit (“PPH”), and methods of use directed to purification of biogas or other raw methane streams. The apparatus includes a homogenizer body, one or more stream inlets (for the raw methane), one or more chilled water inlets, a mixing zone where the water stream is commingled with the raw methane stream, and a venturi immediately downstream from the mixing zone such that the commingled streams are pulled into the venturi resulting in homogenization. The PPH components are insulated to maintain the chilled water of the various streams at a cooled, below ambient temperature, increasing dissolution of the contaminant gases into the chilled water, and producing a purified methane stream including little or no H.sub.2S and CO.sub.2.

Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.

The invention is directed to a device (1) and a process for dissolving a gas into a liquid like carbonating a water based beverage, comprising a pump (4) for the liquid, a mixing venture nozzle (8) with a main inlet (10) fluidly connected to the pump (4), at least one side inlet (14) connectable to a source of pressurized gas (6), and an outlet. The device (1) comprises also a conical flow restrictor (24) fluidly downstream of the mixing venture nozzle (8), and a pipe (20) of a length of at least 0.5 m fluidly interconnected between the mixing venture nozzle (8) and the flow restrictor (24).

A liquid-diffusing filter which can aerate a liquid by diffusing it and thus exposing it to air to oxygenate the liquid. The user can pour a liquid through the device and it comes out diffused. Thus a superior liquid aerating solution is provided that can more simply aerate a beverage.

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

A bubble generating device is disclosed which includes: a bubble generator including a tubular body, a liquid introduction port, a gas introduction port, and a discharge port; and a gas supply unit including a gas supply port, a pressurized gas being supplied to the bubble generator through the gas supply port, wherein the flow passage of the bubble generator extends substantially along a same axis, a plurality of reduced diameter portions each having an inner diameter reduced are provided along a direction along which the liquid flows, and gas-liquid mixing portions are provided downstream of the respective reduced diameter portions in a contiguous manner, each of the gas-liquid mixing portions having an inner diameter larger than a minimum inner diameter of each of the plurality of reduced diameter portions, and the gas introduction port of the bubble generator is formed of a plurality of through holes.