Microfluidic methods for barcoding nucleic acid target molecules to be analyzed, e.g., via nucleic acid sequencing techniques, are provided. Also provided are microfluidic, droplet-based methods of preparing nucleic acid barcodes for use in various barcoding applications. The methods described herein facilitate high-throughput sequencing of nucleic acid target molecules as well as single cell and single virus genomic, transcriptomic, and/or proteomic analysis/profiling. Systems and devices for practicing the subject methods are also provided.

A microfluidic chip and a cosmetic manufacturing apparatus including the microfluidic chip are disclosed. The microfluidic chip contains an inlet portion including a first and a second fluid inlets; a dissolution portion including a first and a second micro flow paths in which a first and a second modular raw materials dissolved by the first and the second fluids are pre-loaded, respectively; a confluence including a third micro flow path in which the first and the second fluids and the first and the second modular raw materials converge; a stir portion including a fourth micro flow path which forms a mixed fluid by mixing the first and the second fluids and the first and the second modular raw materials; and a vortexing portion configured to form a cosmetic composition.

The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.

The invention provides methods and compositions for carbonation of cement mixes using pressurized carbon dioxide delivered to the mix.

[Problem] To provide a device for manufacturing composite particles constructed by flow paths having relatively great widths and capable of controlling an adsorption ratio of particles, and to provide a method for manufacturing composite particles using this manufacturing device.

[Solution] A device for manufacturing composite particles includes at least one first inlet flow path (2) for supplying a first fluid, at least one second inlet flow path (3) for supplying a second fluid, and a mixing flow path (5) for merging the first fluid and the second fluid supplied respectively from the first inlet flow path and the second inlet flow path and allowing the two kinds of fluids to flow down for a predetermined length while mixing the two kinds of fluids. The mixing flow path is a continuous flow path and has a heterogeneous cross-sectional flow path area in a continuity direction thereof.

The disclosure provides for a lab-on-a-chip (LOC) device and a method of fabrication thereof. Additionally, a system and a method for point of care testing of multiple biomarkers such as glucose, cholesterol, creatinine, uric acid, and bilirubin is provided. The microfluidic assembly consists of three layers in which the top and the middle layers are made up of polydimethylsiloxane (PDMS) and the bottom layer with polyethylene terephthalate (PET). The device integrates screen printed non-enzymatic electrochemical sensors in the bottom layer for simultaneous detection of glucose, cholesterol, creatinine, uric acid, and bilirubin. A hand held potentiostat with readout enables readout for the point of care application of integrated sensing device. The device developed has potential to revamp healthcare by providing access to affordable technology for better management a diabetes and related complications at every door step.

The present application relates to an improved apparatus for mixing intensification in multiphase systems, which can be operating in continuous or batch mode. In particular, it relates to a reactor, which can be assembled and disassembled easily for cleaning. The apparatus is based on oscillatory flow mixing (OFM) and comprises an oscillatory flow plate reactor (OFPR) provided with 2D Smooth Periodic Constrictions (2D-SPCs). The apparatus can be fully thermostatized and it is based on a modular system, in order to achieve most of the industrial application. The OFPR is suitable for multiphase applications such as screening reactions, bioprocess, gas-liquid absorption, liquid-liquid extraction, precipitation and crystallization. Regarding its size and geometry and the ability to operate at low flow rates, reagent requirements and waste are significantly reduced, as well as the manufacturing and operating costs, compared to the common reactor, such as continuous stirred tank reactor (CSTR) and the conventional OFR.

Fume extract extracted by a fume-dissolution harvesting and accumulation system for accumulating in a tincture featuring at least one sonic cavitation device configured to exert cavitation effect in a solvent-fume mix, and a fume generating compartment for burning and/or vaporizing fume-releasing source-material configured to produce a portion of the fume at burning temperatures, and another portion at evaporation temperatures. Corresponding method is provided.

An apparatus for mixing fluids, with particular application in the mixing of air and water for facilitating aerobic biological reactions for breaking down organic material. The apparatus includes gas-lift pump effected recirculation through a mixing chamber (in which a toroidal vortex is established) into a reactor vessel having an upper agitation region and a lower separation region. An embodiment for use with compostable materials includes an assembly for handling settleable solids and maintaining suspension of buoyant materials; a foam suppression device and an overflow reservoir.

A microfluidic device includes a substrate, first and second capillary inlets, a microfluidic channel unit, an outlet disposed downstream of the microfluidic channel unit, and a suction member disposed downstream of the outlet. A first liquid is drawn into a first sub-channel and a main channel of the microfluidic channel unit through the first capillary inlet. A second liquid is drawn into a second sub-channel of the microfluidic channel unit through the second capillary inlet. The suction member provides a predetermined suction force to permit the second liquid to penetrate into the first liquid and to break up into droplets in the first liquid, thereby generating monodisperse emulsions.