Provided herein are methods of sterilizing fish by contacting an embryonic or juvenile fish with a compound of formula (I). In some embodiments, the compound is primordazine or a derivative thereof.

Fatty liver was induced by administering agents for inducing organ inflammation to experimental animals to evoke insulin resistance and by rearing them with high-fat diets. As a result, steatohepatitis was successfully induced in the animals. The animals show pathological findings similar to those of humans. By using these model animals, substances for treating or preventing diseases can be efficiently screened and the efficacy of medicinal substances can be effectively evaluated.

Nonsense-mediated mRNA decay (NMD) polypeptides, nucleic acids encoding NMD polypeptides, and methods of using such polypeptides and nucleic acids in the treatment of ALS and in screening for agents for the treatment of ALS are described.

Provided herein are methods of sterilizing fish by contacting an embryonic or juvenile fish with a compound of formula (I). In some embodiments, the compound is primordazine or a derivative thereof.

Nonsense-mediated mRNA decay (NMD) polypeptides, nucleic acids encoding NMD polypeptides, and methods of using such polypeptides and nucleic acids in the treatment of ALS and in screening for agents for the treatment of ALS are described.

Disclosure is a composite mouse model for simulating the exacerbation of periodontitis by hyperuricemia, as well as the establishment methods and applications thereof. The establishment methods includes: intraperitoneally injecting potassium oxonate into a mouse continuously for 7 days to establish a hyperuricemia model; ligating the maxillary second molar of the mouse with a surgical silk thread to construct a periodontitis model; continuously intraperitoneally injecting potassium oxonate into the mouse for another 7 days (days 8 to 14) to establish a composite mouse model that simulates exacerbation of periodontitis by hyperuricemia; continuously intraperitoneally injecting potassium oxonate for 14 days (days 15 to 28) at a fixed frequency once a day to maintain stability of the hyperuricemia model and constructing a stable hyperuricemia compound periodontitis mouse model; and on the 15th day, intraperitoneally injected allopurinol for 14 days continuously to decrease uric acid.

The present disclosure relates to animal models simulating human biliary atresia. The model includes a large animal treated with an agent causing sclerosis of intrahepatic bile ducts in the animal to simulate human bile duct injury.

The invention provides compositions and methods useful for the treatment and prevention of conditions associated with short telomere length, particularly kidney fibrosis.

A method of preparing a mouse model of herpes using a herpes simplex virus and hydrocortisone, and a mouse model of herpes prepared using the method are disclosed. The method makes it possible to obtain an animal model in which symptoms of herpes clearly appear. The obtained animal model can be widely used in the development of therapeutic agents for herpes simplex virus infection. A use of the mouse model in screening and developing a candidate therapeutic agent is also disclosed.

The invention is concerned with a fusion protein comprising interleukin 13 and a regulatory cytokine, for example, an interleukin chosen from interleukin 4, interleukin 10, interleukin 27, interleukin 33, transforming growth factor beta 1, transforming growth factor beta 2, and interleukin 13, a nucleic acid molecule encoding such fusion protein, a vector comprising such nucleic acid molecule, and a host cell comprising such nucleic acid molecule or such vector. The invention further pertains to a method for producing such fusion protein. The fusion protein or a gene therapy vector encoding the fusion protein may be used in the prevention or treatment of a condition characterized by pathological pain, chronic pain, neuro-inflammation and/or or neurodegeneration.