Microcavity arrays and methods for quantitative biochemical and biophysical analysis of populations of biological variants. Examples include high-throughput analysis of cells and protein products use a range of fluorescent assays, including binding-affinity measurement and time-resolved enzyme assays. Laser-based extraction of microcavity contents.

An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

A method and system for heating and/or inspecting a portable microfluidic assay cartridge for performing an assay includes receiving the assay cartridge on a receiving region of a translatable table under automated control, heating the cartridge, during performance of the assay, with a planar radiant heater plate, the heater plate having an aperture through which an inspection axis extends, and/or inspecting the cartridge using an optical system constructed to inspect the cartridge along the inspection axis by reading a fluorescent light signal which passes through the aperture in the heater plate. In addition, the cartridge moves with movement of the translation table, and the heater plate and optical system may be stationary, and the inspection axis may be fixed.

Disclosed herein include systems, apparatuses, devices, and methods for introducing one or more components into a fluid. A first fluid and a second fluid can be co-injected into a fluidic channel of a flow cell. In some embodiments, the first fluid and a second fluid are immiscible (e.g. an aqueous buffer and a non-aqueous liquid). In some embodiments, the second fluid is less dense than the first fluid.

The disclosure pertains to a microfluidic cartridge comprising at least one microchannel and at least a set of functionalized microcarriers, the microcarriers being localized within the microchannel, wherein the functionalized microcarriers are coated with at least a lyoprotectant. The disclosure further pertains to a process of manufacture of a microfluidic cartridge according to the invention, said process comprising: providing a microfluidic cartridge comprising at least one microchannel and at least a set of functionalized microcarriers, preferably in suspension in a buffer solution, the microcarriers being localized within the microchannel; flowing a stabilizing buffer into the at least one microchannel and incubating the functionalized microcarriers with said stabilizing buffer for at least 10 minutes, wherein the stabilizing buffer is a composition comprising a lyoprotectant, preferably wherein the lyoprotectant is chosen from the list consisting of sugars and sugar alcohols and mixtures thereof; and drying the at least one microchannel.

An apparatus and a method are used for investigating the naphtha reforming process in catalyst test devices with reactors arranged in parallel. The apparatus has a plurality of reactors arranged in parallel with reaction chambers (R1, R2, . . . ), a product fluid supply, a process control, and at least one analysis unit. Each individual reactor has an outlet line for the product fluid stream, wherein the analysis unit is operatively connected to each outlet line for the product fluid stream and the apparatus is functionally connected to the control of the apparatus. In carrying out the method, naphtha-containing reactant fluid streams are brought into contact with catalysts in the individual reactors and the product fluid streams are subsequently supplied to the online analysis unit from the respective outlet lines of the individual reactors and analyzed. Using the evaluation of the online analytical characterization data, the process parameters of the respective reactor unit are adapted. The process steps of analytical characterization, evaluation, and adaptation of process parameters are repeated for the duration of the investigation.

A method for forming a lipid membrane vesicle includes: filling a chamber with a first aqueous solution by introducing it to a liquid flow path facing a microreactor chip hydrophobic layer main surface; forming a first lipid monolayer membrane in an opening part of the chamber filled with the solution; forming a second lipid monolayer membrane on a layer interface of the organic solvent formed on the main surface of the hydrophobic layer with a second aqueous solution by introducing the solution to the liquid flow path; allowing a first aqueous solution form in the chamber to alter to a spherical droplet covered with the first lipid monolayer membrane; and forming a lipid membrane vesicle by moving the droplet to a position of the second lipid monolayer membrane by applying a physical action, and by zipping the first lipid monolayer membrane covering the droplet and the second lipid monolayer membrane.

A synthesis device comprises a plurality of pipes, a feeding unit, a reaction vessel, and a measurement mechanism. The pipes extend from a plurality of storage containers, respectively, in which a plurality of types of solutions are stored. The feeding unit is configured to feed the solutions in the storage containers through the pipes. The solutions selectively fed from the storage containers are put in the reaction vessel to generate a synthesized product by chemical synthesis. The measuring mechanism is provided between the storage containers and the reaction vessel in a middle of an overall flow path including the pipes, the measuring mechanism being configured to measure the solutions fed to the reaction vessel.

The invention relates to a flow element for transferring fluids comprising a capillary cartridge (1) having an integrated capillary line (3). The capillary cartridge according to the invention (1) has a ring-shaped channel (8) and securing grooves (6, 6), wherein the flow element is characterized in that the capillary line (3) is arranged in the ring-shaped channel (8). The ends of the capillary lines (3) are connected to connection elements (9) in which securing grooves (6, 6) are secured in a positive locking manner. The flow elements according to the invention contribute toward improved manageability and effectiveness of components. In a preferred embodiment, the flow elements are used as a distribution system in the form of a plurality of capillary cartridges (1-1, 1-2, . . . ). Such distribution systems are of technical importance in the field of catalyst testing apparatuses with reactors arranged in parallel.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.