Apparatuses and a method for plate-based oligonucleotide synthesis are disclosed. In one example, an apparatus used in oligonucleotide synthesis includes a machined block to receive a commercially-available synthesis plate. A keeper is used to apply pressure to the commercially-available synthesis plate, and a sealing element is used to seal the commercially-available synthesis plate to the machined block. Other methods and apparatuses are disclosed.

Complete nucleic acid library preparation devices are provided. Aspects of the devices include: a thermal chip module comprising multiple nodes; one or more plate locations; a robotically controlled liquid handler configured to transfer liquid between the one or more plate locations and the thermal chip module; and a bulk reagent dispenser configured to access each node of the thermal chip module.

Chemical synthesizer systems and methods for operating the same. One method includes receiving a first queue of instructions including a plurality of delivery instructions for operating a delivery assembly with respect to a plurality of synthesis plates and a plurality of vacuum instructions, grouped in a plurality of vacuum sections, for operating a vacuum assembly with respect to the plurality of synthesis plates. The method also includes sequentially processing each instruction included in the first queue of instructions by (i) executing the instruction when the instruction is one of the plurality of delivery instructions and (ii) moving, when the instruction is one of the plurality of vacuum instructions, one of the plurality of vacuum sections including the instruction to a second queue of instructions and executing instructions included in the second queue of instruction in parallel with instructions included in the first queue of instructions.

A microreactor array platform and method for sealing a reagent in microreactors of an array of microreactors are provided. The microreactor array platform includes an array of microreactors, and a sealing film having a first surface and an opposite second surface, the sealing film configured to movably seal the array of microreactors. The microreactor array platform also includes an injector for delivering a reagent into the array of microreactors via a fluid path between the array and the second surface of the sealing film, and an applicator for directing a sealing liquid against the first surface of the sealing film. The microreactor array platform further includes a system for creating a pressure differential between the reagent in the injector and a space between the array of microreactors and the second surface of the sealing film.

Apparatuses and a method for plate-based oligonucleotide synthesis are disclosed. In one example, an apparatus used in oligonucleotide synthesis includes a machined block to receive a commercially-available synthesis plate. A keeper is used to apply pressure to the commercially-available synthesis plate, and a sealing element is used to seal the commercially-available synthesis plate to the machined block. Other methods and apparatuses are disclosed.

Apparatuses and a method for plate-based oligonucleotide synthesis are disclosed. In one example, an apparatus used in oligonucleotide synthesis includes a synthesis adapter base to receive a commercially available synthesis plate. A keeper is used to apply pressure to the commercially available synthesis plate, and a sealing element is used to seal the commercially-available synthesis plate to the system adapter base. Other methods and apparatuses are disclosed.