Provided in some aspects are methods of patterning a surface in situ for producing an array on the surface, for example, by partitioning of beads comprising oligonucleotides into spatially predefined regions, to generate unique DNA sequences in spatial positions in the array. Compositions such as nucleic acid arrays produced by the methods are also disclosed.

Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photocontrollable ligation of nucleic acid molecules with splints, wherein at least one of the splints comprises a photo-crosslinkable moiety. Photo-induced crosslinking of the splint to oligonucleotide molecules immobilized on a substrate allows the splint to remain attached to the oligonucleotide molecules during subsequent rounds of photoresist application and removal. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

Complete nucleic acid library preparation devices are provided. Aspects of the devices include: a thermal chip module comprising multiple CLC reaction wells; one or more plate locations; a robotically controlled liquid handler configured to transfer liquid between the one or more plate locations and the thermal chip module; and a bulk reagent dispenser configured to access each CLC reaction well of the thermal chip module.

A method for manufacturing a chip for analyzing nucleic acid information of a biological sample. The chip is suitable for analyzing spatial transcriptomic information of a biological tissue sample. Also provided are the chip for analyzing the nucleic acid information of the biological sample and a method for using the chip to analyze the spatial transcriptomic information of the biological tissue sample. Nucleic acid expression information in cells of the tissue sample can be effectively obtained, and comprises spatial omics information.

The invention relates to a recognizable carrier for determining physical, chemical or biochemical interactions by means of optical measurement methods. The carrier comprises a surface that defines a substrate surface and that has a base layer coated with reactive elements, which are bonded to receptor molecules, wherein the base layer and/or the reactive elements are provided with a pattern of holes which forms a code and/or the reactive elements are provided with linker molecules or markers which form a code. The substrate surface may additionally have a macroscopically planar pattern which is applied using laser light or chemical etching and forms a code. The invention likewise relates to a method for producing a recognizable carrier for spectroscopic processes and/or intensiometric tests to determine said interactions. The code to recognize the carrier can be controlled via a read-out unit coupled to the photometric analysis unit. Such a carrier can be used to analyze biomolecules during security checks, access controls or in-vitro diagnostics.

Provided is a method of magnetically controlling a magnetic structure, the method including: providing a solution containing magnetic structures, each including a magnetic axis in which magnetic nanoparticles are arranged; and controlling movements of the magnetic structures by applying an external magnetic field to the solution.

Complete nucleic acid library preparation devices are provided. Aspects of the devices include: a thermal chip module comprising multiple CLC reaction wells; one or more plate locations; a robotically controlled liquid handler configured to transfer liquid between the one or more plate locations and the thermal chip module; and a bulk reagent dispenser configured to access each CLC reaction well of the thermal chip module.

A method and an array filling system for loading a plurality of disparate sample containers, the sample containers comprising an integral structure. Each receptacle is characterized by a hydrophilic surface,, and the receptacles are separated by a hydrophobic surface. The system has a liquid transfer device capable of holding liquid and adapted for motion to cause sequential communication of liquid held in the liquid transfer device with successive receptacles of the array by dragging the liquid across the hydrophobic surface.

Disclosed herein include methods, devices, kits, and systems for nucleic acid sequencing, for example, to determine cell-cell interaction using a dielectrophoresis microfluidic device.