Particles comprising a plant protein shell and encapsulating hydrophobic compounds and optionally also bioavailability enhancers, are provided. Further provided is a composition and a kit comprising the particle and methods of preparation thereof. Methods of use, such as for enhancing the bioavailability of the hydrophobic compound, are also provided.

Provided herein are methods of encapsulating molecules in milk fat globules and oleosomes, and compositions and pharmaceutical formulations of compositions with molecules encapsulated in milk fat globules and oleosomes.

Provided herein are methods of encapsulating molecules in milk fat globules and oleosomes, and compositions and pharmaceutical formulations of compositions with molecules encapsulated in milk fat globules and oleosomes.

The present invention describes pulsed release caffeine formulations comprising caffeine particulate systems prepared by using release retarding agents and at least one or more food grade excipients. The present invention also relates to the process for preparation of caffeine formulations, wherein plurality of particulate systems combined to get the pulsed release caffeine formulation comprising release retarding agents, which provide caffeine release in pulses at different physiological conditions. The pulsed release caffeine formulation is prepared as tablets, capsules, soft gel capsules, caplets, oil suspensions or mixed with nutrient supplements for administration to the subjects. Caffeine formulations as described herein release the caffeine over entire GIT in pulses in a period of 2 to 8 hours and can be administered to subjects for boosting energy, improving concentration, cardiovascular health and for weight management.

The present invention describes pulsed release caffeine formulations comprising caffeine particulate systems prepared by using release retarding agents and at least one or more food grade excipients. The present invention also relates to the process for preparation of caffeine formulations, wherein plurality of particulate systems combined to get the pulsed release caffeine formulation comprising release retarding agents, which provide caffeine release in pulses at different physiological conditions. The pulsed release caffeine formulation is prepared as tablets, capsules, soft gel capsules, caplets, oil suspensions or mixed with nutrient supplements for administration to the subjects. Caffeine formulations as described herein release the caffeine over entire GIT in pulses in a period of 2 to 8 hours and can be administered to subjects for boosting energy, improving concentration, cardiovascular health and for weight management.

A process for producing a fat-encapsulated ingredient. A fat is metered into an extruder, the fat having a melting point of at least 50° C. The fat is heated in in the extruder during rotation of at least one screw. At least one ingredient is metered into a barrel of the extruder to produce a flowable mixture. The flowable mixture is cooled in a a downstream adjacent section of the extruder barrel. The mixture is subsequently discharged through an extruder die. After discharge, the mixture is comminuted.

A process for producing a fat-encapsulated ingredient. A fat is metered into an extruder, the fat having a melting point of at least 50° C. The fat is heated in in the extruder during rotation of at least one screw. At least one ingredient is metered into a barrel of the extruder to produce a flowable mixture. The flowable mixture is cooled in a a downstream adjacent section of the extruder barrel. The mixture is subsequently discharged through an extruder die. After discharge, the mixture is comminuted.

A process for the production of fat-encapsulated follistatin. The process includes metering fat having a melting point of at least 40° C. into an extruder, heating the fat in the extruder during rotation of the at least one screw, metering the follistatin into the extruder barrel to produce a flowable mixture, cooling the flowable mixture in a downstream adjacent section of the extruder barrel, subsequently discharging the mixture through an extruder die, and comminuting the mixture after it discharges from the extruder die.

A process for the production of fat-encapsulated follistatin. The process includes metering fat having a melting point of at least 40° C. into an extruder, heating the fat in the extruder during rotation of the at least one screw, metering the follistatin into the extruder barrel to produce a flowable mixture, cooling the flowable mixture in a downstream adjacent section of the extruder barrel, subsequently discharging the mixture through an extruder die, and comminuting the mixture after it discharges from the extruder die.

The present invention relates to a method of manufacturing spray-dried powders. During the process, a solvent is used. The process is done batchwise such that the emulsification mass ratio is low when removal of the solvent is started. Preferred solvents are isopropyl acetate and ethyl acetate. The invention also relates to a set-up to run the inventive process at industrial scale.