The disclosure includes a method of introducing a polynucleotide into a male germ cell or a Sertoli cell, comprising injecting an adeno-associated virus vector comprising the polynucleotide into the testis of a vertebrate.

The present invention, in some embodiments thereof, is directed to a method for selecting a gender of an avian fertilized unhatched egg obtained from the crossing of a transgenic male avian subject and a transgenic female avian subject. Further provided is a kit for preparing a transgenic avian subject.

The present invention relates to means and methods for increasing the efficiency of recombinant protein expression and, more particularly, to means and methods for optimizing the industrial production of recombinant proteins in pyralid moths eggs (which are insect pests of stored foods), especially Mediterranean flour moth eggs (Ephestia kuehniella). Furthermore, the present invention relates to the pyralid moths eggs itself which contains a recombinant baculovirus and, infection of recombinant baculovirus into pyralid moths eggs and, transformation or transduction or transfection by recombinant baculoviruses or bacmids. In addition, it relates to an appropriate device for carrying out the method of the present invention.

The present invention has found that chimeric animals suffer from noticeable inflammation after birth, though neither immune response nor inflammation in the fetal period of these animals has been reported hitherto. This is an unexpected finding since chimeric animals in the fetal period were exclusively analyzed in prior studies and thus it is deemed that immunotolerance has been theoretically established therein. The present invention provides a composition for suppressing immune response or inflammation in the fetal period of a born chimeric animal.

Described herein are embodiments relating to manipulation of populations and sex ratio in populations through DNA sequence modifications.

Provided herein is a mouse that produces hybrid antibodies containing human variable regions and mouse constant regions.

The present invention relates to a method for the generation of V.sub.H heavy chain-only antibodies in a transgenic non-human mammal. In particular, the present invention relates to a method for the production of a V.sub.H heavy chain-only antibody in a transgenic non-human mammal comprising the step of expressing more than one heterologous V.sub.H heavy chain locus in that mammal.

The receptor for Interleukin 35 (IL-35) is provided. The Interleukin 35 Receptor (IL-35R) comprises a heterodimeric complex of the Interleukin12R2 receptor and the gp130 receptor. Various compositions comprising the IL-35R complex, along with polynucleotides encoding the same and kits and methods for the detection of the same the same are provided. Methods of modulating the activity of IL-35R or modulating effector T cell functions are also provided. Such methods employ various IL-35R antagonists and agonists that modulate the activity of the IL-35R complex and, in some embodiments, modulate effector T cell function. Further provided are methods for screening for IL-35R binding agents and for IL-35R modulating agents. Various methods of treatment are further provided.

The invention relates to newly characterized light-inducible inward proton pumps and their use in medicine, their utility as optogenetic tools, nucleic acid constructs encoding same, expression vectors carrying the nucleic acid construct, cells comprising said nucleic acid construct or expression vector, and their respective uses.

Method for controlling for the appearance of seizures in the mammalian brain comprising modifying the abundance of a specific miRNA-miR-211, for uses in preventing seizures and providing a model system to examine the effect of a drug or a treatment to seizures.