A cutting and threshing blade for a bush hog, wherein the blade has a trailing edge and a leading edge. At least a third of the leading edge of the upper-side of an elongate distal portion of the blade has a knife edge with a knife length that thickens toward the trailing edge. The trailing edge can have an upward air deflector. The bottom-side of the elongate distal portion has a threshing element, which includes a threshing block, which is a steel block having a block length that is comparable to the knife length and projects downward and substantially parallel to the knife edge. The rotating blades pummel growth carriers (i.e.; cob, husk) bearing seeds, grains, and kernels with enough force that they are released. The synergistic rotating knife edge and threshing block transform the bush hogging process from a cutting process to a cutting and threshing process.

The present invention relates to a new cocktail of bacteriophages with specific lytic activity against Salmonella enteritidis, Salmonella typhimurium and Salmonella paratyphi B., for reducing, eliminating and/or preventing them in farm animals and animals from the poultry sector, such as poultry, hens and breeding hens, in addition to eggs. It may be administered as an additive in the feed, in water or by spray. Moreover, the cocktail may be used as a disinfectant in work areas of farms and abattoirs, and in processed foods, without affecting the organoleptic properties of the product.

The present invention relates to methods of gender determination and identification in avian subjects. More specifically, the invention provides non-invasive methods using transgenic avian animals that comprise at least one reporter gene integrated into at least one gender chromosome Z or W. The transgenic avian animals of the invention are used for gender determination and selection of embryos in unhatched avian eggs.

Embodiments of the disclosure encompass systems that utilize chick chorioallantoic membranes (CAM) as models for cancer xenografts, including at least patient-derived xenografts (CAM-PDX). In particular embodiments, the system employs the CAM-PDX to graft and culture different types of tumor tissue on a single or multiple eggs. In specific embodiments multiple tumor regions of a single tumor are cultured on a single egg. Frozen tissue is successfully revived, in specific embodiments. Downstream applications following successful establishment of CAM-PDX models are encompassed.

A transgenic avian containing in its genome an exogenous nucleotide sequence which includes a promoter component and a vector with reduced promoter interference wherein the exogenous nucleotide sequence is integrated into the genome and the avian.

The present invention relates to methods for producing a non-human animal, such as an avian, comprising a targeted germline genetic modification wherein a nuclease, which may be CRISPR, ZFN or TALEN, is delivered to spermatozoa prior to oocyte fertilization.

Provided are a poultry knock-in egg and knock-out egg. The present invention pertains to a knock-out poultry egg in which at least one oviduct-specific gene has been knocked out, said gene being selected from the group consisting of ovalbumin, ovomucoid, ovomucin, ovotransferrin, ovoinhibitor, and lysozyme, and at least one egg allergen protein has been reduced or eliminated, said protein being selected from the group consisting of ovalbumin, ovomucoid, ovomucin, ovotransferrin, ovoinhibitor, and lysozyme.

A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

The invention concerns a gallinacean embryo in which cancer cells have been grafted within the embryo tissue, characterised in that the embryo is at a developmental stage between the HH10 and HH25 stages at the time of the grafting, wherein said cancer cells are not neuroblastoma cells and said cells form tumors inside the embryo.

The invention comprises systems and methods for controlling environmental conditions within and/or around an animal enclosure. The systems and methods preferably comprise a climate control system and infrared sensors in a poultry house having a first climate and housing poultry having one or more measurable parameters such a temperature. The infrared sensors detect infrared light information emitted by a head and/or vent of poultry in the house. The climate control system processes the information to calculate an internal temperature of poultry and activates one or more climate conditioning devices in the house to change the first climate to a second climate determined by the detected information and/or calculated internal temperatures of the poultry. The systems and methods may be deployed in houses comprising thousands of poultry and one or more climate zones. The systems and methods may make one or more climate changes based on one or more different parameters.