The invention comprises systems and methods for controlling environmental conditions within and/or around an animal enclosure. The systems and methods preferably comprise a climate control system and infrared sensors in a poultry house having a first climate and housing poultry having one or more measurable parameters such a temperature. The infrared sensors detect infrared light information emitted by a head and/or vent of poultry in the house. The climate control system processes the information to calculate an internal temperature of poultry and activates one or more climate conditioning devices in the house to change the first climate to a second climate determined by the detected information and/or calculated internal temperatures of the poultry. The systems and methods may be deployed in houses comprising thousands of poultry and one or more climate zones. The systems and methods may make one or more climate changes based on one or more different parameters.

Provided are a poultry knock-in egg and knock-out egg. The present invention pertains to a knock-out poultry egg in which at least one oviduct-specific gene has been knocked out, said gene being selected from the group consisting of ovalbumin, ovomucoid, ovomucin, ovotransferrin, ovoinhibitor, and lysozyme, and at least one egg allergen protein has been reduced or eliminated, said protein being selected from the group consisting of ovalbumin, ovomucoid, ovomucin, ovotransferrin, ovoinhibitor, and lysozyme.

This disclosure provides, among other things, a transgenic animal that uses gene conversion for antibody diversification, comprising B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding an autonomous heavy chain (AHC) variable domain; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the AHC variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

Provided herein is a transgenic chicken comprising a genome having an endogenous immunoglobulin heavy chain gene in which the IgY CH1 coding sequence is functionally deleted. In certain embodiments, in B cells of the chicken the endogenous immunoglobulin heavy chain gene comprises: (a) a functional immunoglobulin heavy chain gene comprising, in operable linkage, a nucleic acid encoding an antibody heavy chain variable domain and a constant region coding sequence in which the IgY CH1 coding sequence is functionally deleted; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the variable domain of (a), wherein the pseudogenes are upstream of the nucleic acid encoding an antibody heavy chain variable domain of (a).

This disclosure provides, among other things, a transgenic animal that uses gene conversion for antibody diversification comprising B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a human heavy chain variable domain; and (b) a plurality of pseudogenes that are operably linked to the functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the human heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene and encode variable domains that have camelizing amino acid substitutions.

A method for preparing an ammonia abatement additive for poultry litter that is formulated to assure activation in an interior environment having various relative humidity conditions. The method includes obtaining a blended litter amendment composition that, based on weight, is about 40% deliquescent salt and about 60% aluminum sulfate, relative to a total weight of the two components. The composition can be made by the user or received, already blended, from a third party. To this blended composition that has been obtained, additional deliquescent salt is added in an amount such that the total amount of deliquescent salt is effective to activate the alum-containing ammonia abatement additive at the targeted low relative humidity condition in the interior of a poultry house. The composition is a sub-component of the ammonia abatement additive which may or may not contain additional compounds. The specified relative proportions (i.e., 40% deliquescent salt and 60% alum) only consider the proportion of deliquescent salt and the proportion of alum relative to the sum of the two components, based on weight. If there are other, extraneous compounds included in the ammonia abatement additive, those extraneous compounds are not counted in the defined and claimed proportions.

A method for preparing an ammonia abatement additive for poultry litter that is formulated to assure activation in an interior environment having various relative humidity conditions. The method includes obtaining a blended litter amendment composition that, based on weight, is about 40% deliquescent salt and about 60% aluminum sulfate, relative to a total weight of the two components. The composition can be made by the user or received, already blended, from a third party. To this blended composition that has been obtained, additional deliquescent salt is added in an amount such that the total amount of deliquescent salt is effective to activate the alum-containing ammonia abatement additive at the targeted low relative humidity condition in the interior of a poultry house. The composition is a sub-component of the ammonia abatement additive which may or may not contain additional compounds. The specified relative proportions (i.e., 40% deliquescent salt and 60% alum) only consider the proportion of deliquescent salt and the proportion of alum relative to the sum of the two components, based on weight. If there are other, extraneous compounds included in the ammonia abatement additive, those extraneous compounds are not counted in the defined and claimed proportions.

The present invention provides compositions of recombinant human lysosomal acid lipase having particular glycosylation patterns for internalization into target cells, a vector containing the nucleic acid encoding human lysosomal acid lipase, a host cell transformed with the vector, pharmaceutical compositions comprising the recombinant human lysosomal acid lipase and method of treating conditions associated with lysosomal acid lipase deficiency.

This disclosure provides, among other things, a transgenic animal and a method of using the same to make antibodies that have a common light chain. In certain embodiments, the transgenic animal may comprising a genome comprising a common light chain transgene, wherein the common light chain transgene comprises a non-immunoglobulin light-chain promoter and a common light-chain coding sequence. In certain embodiments, the common light chain is constitutively expressed.