The present invention relates to methods for transfecting cells. In particular, the present invention relates to methods of transfecting primordial germ cells in avians, and to methods of breeding avians with modified traits.

A transgenic chicken having a genome comprising a modified immunoglobulin heavy chain (IgH) locus is provided. The locus lacks the entire contiguous endogenous chicken V-D-J region and contains a human VH segment, a human D cluster, a human J segment and a plurality of upstream pseudogenes based on human VH sequences. The modified IgH locus undergoes V(D)J recombination in the chicken and the chicken produces antibodies that have a diversified immunoglobulin heavy chain.

A breeding method for improving reproductive performance of a chicken specialized dam line includes the steps of forming an F1 generation group, forming a breeding group, screening a breeding group, forming an F2 generation group, and continuously breeding to an Fn generation group. The method includes raising in small-scale groups and natural mating for systematic selection and breeding, which effectively reduces the generation interval and has the features of easy operation and fast genetic progress, not only ensuring animal welfare, but also effectively improving production performance.

The present invention provides compositions of recombinant human lysosomal acid lipase having particular glycosylation patterns for internalization into target cells, a vector containing the nucleic acid encoding human lysosomal acid lipase, a host cell transformed with the vector, pharmaceutical compositions comprising the recombinant human lysosomal acid lipase and method of treating conditions associated with lysosomal acid lipase deficiency.

The present invention provides systems and non-invasive methods for gender selection of eukaryotic organisms More specifically, the invention applies the CRISPR-Cas system as well as any derivatives and fusion proteins thereof for creation of transgenic eukaryotic organisms and for selecting the desired gender of the resulting progeny.

A transgenic chicken comprising an inactivated heavy immunoglobulin gene and/or inactivated light chain immunoglobulin gene is provided, as well as cells and targeting vectors for making the same.

The invention relates to eye-friendly green-blue mixed LED lighting methods and systems for chicken houses. In a chicken house, an evenly-distributed cyan light environment may be created for rearing broiler chickens. The chicken house may include mixed LED lamps associated with the cyan light environment, and the mixed LED lamp may be formed by combining green LED beads and blue LED beads on a lighting fixture with a mixing proportion of from about 0.2 to about 5. The mixed LED lamps may be used for about 24-hour lighting in the first three days of the breeding period. From the fourth day of the breeding period, the daily lighting duration may be progressively decreased by about one hour every day. The mixed LED lamps may be evenly arranged in the chicken house to form a square array.

The present invention relates to methods of fertilization and gender determination and identification in avian subjects. More specifically, the invention provides non-invasive methods using transgenic avian animals that comprise at least one reporter gene, specifically, RFP, integrated into at least one gender chromosome Z or W. The transgenic avian animals of the invention are used for gender determination and selection of embryos in unhatched avian eggs.

A cutting and threshing blade for a bush hog, wherein the blade has a trailing edge and a leading edge. At least a third of the leading edge of the upper-side of an elongate distal portion of the blade has a knife edge with a knife length that thickens toward the trailing edge. The trailing edge can have an upward air deflector. The bottom-side of the elongate distal portion has a threshing element, which includes a threshing block, which is a steel block having a block length that is comparable to the knife length and projects downward and substantially parallel to the knife edge. The rotating blades pummel growth carriers (i.e.; cob, husk) bearing seeds, grains, and kernels with enough force that they are released. The synergistic rotating knife edge and threshing block transform the bush hogging process from a cutting process to a cutting and threshing process.

A transgenic non-human animal is provided. In certain embodiments, the animal comprises a genome comprising an immunoglobulin heavy chain locus comprising: a) a transcribed gene encoding a fusion protein comprising, from N-terminus to C-terminus: i. a scaffold comprising a first binding domain; and ii. a heavy chain constant region operably linked to the scaffold; wherein the scaffold is capable of specifically binding to a target in the absence of additional polypeptides; and b) a plurality of pseudogenes that are operably linked to the transcribed gene and that donate, by gene conversion, nucleotide sequence to the part of the transcribed gene that encodes the binding domain.