A fusion protein including a LC3 protein which includes a first tag and a protein which includes a second tag.

Protein indicators useful for calcium imaging, in particular, red genetically-encoded calcium indicators (GECIs) disclosed herein rival best-of-class green GECIs in terms of sensitivity for detecting neural activity, and can be monitored in vivo. The presently-disclosed subject matter further includes a method of monitoring cell activity comprising stimulating a cell comprising a red GECI polypeptide; and detecting fluorescence emitted by the cell.

A display apparatus includes a transparent electronic display such as a non-backlit LCD screen, a display area disposed behind the transparent electronic display, and a light source for illuminating the display area. When the light source is activated, the pixels of the LCD screen, and objects behind the screen, become visible. Various examples of the display apparatus include additional enhancements to the visual image and/or functionality. These enhancements include additional monitors for coordinated displays, user-controlled turntables with coordinated informational displays, parallax compensation, RFID sensors for identifying objects to be displayed, and switchable liquid crystal films and/or polarized mirror coatings to further control revealing and concealing of objects behind the screen. Some examples of the display apparatus may include an animal habitat. The animal habitat may be selectively revealed or concealed. The display apparatus may incorporate screen displays with animal movements for unique visual effects.

A multi-segmented, soft-bodied, artificial fishing lure or swim bait with a natural profile includes plastic control planes in each body segment to generate a lure that is stable in the water, and demonstrates realistic swimming motions in a range of retrieve speeds and even when allowed to sink. At least one clear, stiff plastic control plane is inserted in each body segment to generate a lifelike swimming motion when the lure is retrieved. Each control plane may be moved within a range of positions to provide realistic motions for specific fishing techniques. The control planes inserted into and sometimes through the lure body are invisible in the water and are adjustable to accommodate a range of water conditions, densities and temperatures. The placement of control planes create the ability to limit unnatural/undesirable movements and expand or accentuate natural and desirable movements in each segment of the body of the lure.

Provided herein are methods of sterilizing fish by contacting an embryonic or juvenile fish with a compound of formula (I). In some embodiments, the compound is primordazine or a derivative thereof.

Among the various aspects herein is the provision of compositions and methods for modulating MYCT1. Methods are provided for regulating tumor angiogenesis (anti-angiogenesis, Myct1-targeted vascular control) and/or immunostimulation, which inhibit tumor growth, in a subject. Methods are provided to quantify MYCT1 to predict responsiveness of a subject having a cancer or tumor to a treatment, guide treatment decisions, select subjects for clinical trials, and evaluate the clinical efficacy of certain therapeutic interventions.

The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

The transplant fish production method includes selecting a donor fish species, selecting first and second fish species which are a combination that could be sterile, producing a hybrid fish species of the selected first fish species and second fish species, and transplanting reproductive cells of the donor fish species into the produced hybrid fish species.

The present invention relates to a pharmaceutical composition containing, as an active ingredient, chemokine-like samdori2 (Sam2) for preventing and treating a mental disease associated with a brain nervous system. By constructing a Sam2-specific knockout zebrafish (wherein, the Sam 2 is expressed in a habenular nucleus) and finding that, through novel tank, scototaxis, and social cohesion tests, the zebrafish shows significantly higher fear and anxiety behaviors than the control; inhibitory neuron expression in neurons overexpressing Sam2 gene is decreased; and a patient due to sam2 gene loss shows autism and the hyperanxiety symptom, the Samdori2 can be usefully applied as an active ingredient for a pharmaceutical composition for preventing and treating a mental disease associated with a brain nervous system.

The present invention relates to transgenic ornamental barbs, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic barbs and methods of providing them to the ornamental fish industry for the purpose of marketing.